Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
FoxO1 mediates an autofeedback loop regulating SIRT1 expression. Xiong S, Salazar G, Patrushev N, Alexander RW. The Journal of biological chemistry. 2011 286:5289-99.
Integration of -catenin, sirtuin, and FOXO signaling protects from mutant huntingtin toxicity. Parker JA, Vazquez-Manrique RP, Tourette C, Farina F, Offner N, Mukhopadhyay A, Orfila AM, Darbois A, Menet S, Tissenbaum HA, Neri C. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2012 32:12630-40.
SIRT3 Antibody, HSIRT3 Antibody, Sir2-like 3 Antibody, SIR2-like protein 3 Antibody, SIR2L3 Antibody, Sirtuin 3 Antibody, Sirtuin type 3 Antibody
SIRT3 / Sirtuin 3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of SIRT3 antibody in Y79 cell line lysates (35 ug/lane). SIRT3 (arrow) was detected using the purified antibody.
Sirt3 Antibody (G265) western blot of mouse kidney tissue lysates (35 ug/lane). The Sirt3 antibody detected the Sirt3 protein (arrow).
Western blot of lysates from 293, HepG2 cell line (from left to right), using SIRT3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
SIRT3 Antibody western blot of 293 cell lysate (35 ug/lane). This demonstrates that the SIRT3 antibody detected SIRT3 protein (arrow).
SIRT3 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The SIRT3 antibody detected the SIRT3 protein (arrow).
SIRT3 Antibody western blot of KG-1 cell line lysates (35 ug/lane). The SIRT3 antibody detected the SIRT3 protein (arrow).