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Anti-SIRT3 / Sirtuin 3 Antibody (aa250-279) LS-C100489

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SIRT3 / Sirtuin 3 Antibodies

Anti-SIRT3 / Sirtuin 3 Antibody (C-Terminus) LS-C46967
Rabbit Polyclonal to Mouse SIRT3 / Sirtuin 3
Mouse
Western blot, ELISA
Unconjugated
Anti-SIRT3 / Sirtuin 3 Antibody (aa170-183) LS-C139812
Goat Polyclonal to Mouse SIRT3 / Sirtuin 3
Mouse, Rat, Pig
Western blot, ELISA
Unconjugated
Western blot Image
Anti-SIRT3 / Sirtuin 3 Antibody (C-Terminus) IHC-plus™ LS-B8047
Rabbit Polyclonal (IgG) to Human SIRT3 / Sirtuin 3
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Rabbit Polyclonal to Human SIRT3 / Sirtuin 3
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human SIRT3 / Sirtuin 3
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)

  • IHC - Paraffin

Specificity and Use

SIRT3 / Sirtuin 3 antibody was raised against kLH-conjugated synthetic peptide between amino acids 250-279 from C-terminal region of human SIRT3.
LS-E8146 - Lyophilized - 100 µg - $145.00
aa250-279

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (2)

1
FoxO1 mediates an autofeedback loop regulating SIRT1 expression. Xiong S, Salazar G, Patrushev N, Alexander RW. The Journal of biological chemistry. 2011 286:5289-99. [PubMed:21149440] [PMC:PMC3037641]
2
Integration of -catenin, sirtuin, and FOXO signaling protects from mutant huntingtin toxicity. Parker JA, Vazquez-Manrique RP, Tourette C, Farina F, Offner N, Mukhopadhyay A, Orfila AM, Darbois A, Menet S, Tissenbaum HA, Neri C. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2012 32:12630-40. [PubMed:22956852]

About SIRT3 / Sirtuin 3

Q9NTG7 NM_012239 NP_036371.1

SIRT3 Antibody, HSIRT3 Antibody, Sir2-like 3 Antibody, SIR2-like protein 3 Antibody, SIR2L3 Antibody, Sirtuin 3 Antibody, Sirtuin type 3 Antibody

SIRT3 / Sirtuin 3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

Western blot
Western blot of SIRT3 antibody in Y79 cell line lysates (35 ug/lane). SIRT3 (arrow) was detected using the purified antibody.

Western blot

Western blot
Sirt3 Antibody (G265) western blot of mouse kidney tissue lysates (35 ug/lane). The Sirt3 antibody detected the Sirt3 protein (arrow).

Western blot

Western blot
Western blot of lysates from 293, HepG2 cell line (from left to right), using SIRT3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot

Western blot
SIRT3 Antibody western blot of 293 cell lysate (35 ug/lane). This demonstrates that the SIRT3 antibody detected SIRT3 protein (arrow).

Western blot

Western blot
SIRT3 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The SIRT3 antibody detected the SIRT3 protein (arrow).

Western blot

Western blot
SIRT3 Antibody western blot of KG-1 cell line lysates (35 ug/lane). The SIRT3 antibody detected the SIRT3 protein (arrow).
 

Requested From: United States
Date Requested: 12/10/2016

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