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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C139812-100 100 µg (0.5 mg/ml) $405 
SIRT3 / Sirtuin 3 Antibody - SIRT3 / Sirtuin 3 antibody immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
SIRT3 / Sirtuin 3 Antibody - SIRT3 / Sirtuin 3 antibody (0.3µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
SIRT3 / Sirtuin 3 Antibody - SIRT3 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SIRT3 / Sirtuin 3 Antibody - SIRT3 / Sirtuin 3 antibody flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton, showing a low level of staining. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).
SIRT3 / Sirtuin 3 Antibody - SIRT3 / Sirtuin 3 antibody immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
SIRT3 / Sirtuin 3 Antibody - SIRT3 / Sirtuin 3 antibody (0.3µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
SIRT3 / Sirtuin 3 Antibody - SIRT3 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
SIRT3 / Sirtuin 3 Antibody - SIRT3 / Sirtuin 3 antibody flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton, showing a low level of staining. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).
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Polyclonal Goat anti‑Mouse SIRT3 / Sirtuin 3 Antibody (aa170‑183, IF, WB) LS‑C139812

Polyclonal Goat anti‑Mouse SIRT3 / Sirtuin 3 Antibody (aa170‑183, IF, WB) LS‑C139812

Antibody:
SIRT3 / Sirtuin 3 Goat anti-Mouse Polyclonal (aa170-183) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Mouse, Rat, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$405
LS-C139812-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
SIRT3 / Sirtuin 3 Goat anti-Mouse Polyclonal (aa170-183) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Mouse, Rat, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
Sirtuin 3 antibody LS-C139812 is an unconjugated goat polyclonal antibody to Sirtuin 3 (SIRT3) (aa170-183) from mouse. It is reactive with mouse, rat and pig. Validated for IF, Peptide-ELISA and WB.
Target
Mouse SIRT3 / Sirtuin 3
Synonyms
SIRT3 | HSIRT3 | Sir2-like 3 | SIR2-like protein 3 | SIR2L3 | Sirtuin 3 | Sirtuin type 3
Host
Goat
Reactivity
Mouse, Rat, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-RASGIPASKLVEAH, from the internal region of the protein sequence according to NP_071878.2NP_001171275.1.
Blocking Peptide
LS-E27704 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C139812. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa170-183
Specificity
Mouse SIRT3. This antibody is expected to recognize both reported isoforms (NP_071878.2; NP_001171275.1). Reported variants represent identical proteins: NP_071878.2; NP_001120823.1
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 30kD band observed in Mouse Heart lysates (calculated MW of 28.8kD according to NP_071878.2). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 15kD. This band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SIRT3 / Sirtuin 3
Q9NTG7 NM_012239 NP_036371.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/5/2020