Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 1A5] (IgG1) to Human SIGLEC5 + SIGLEC14
Immunofluorescence, Immunoprecipitation, Flow Cytometry, ELISA (applications tested for the base form of this product only)
Human SIGLEC5 + SIGLEC14
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [1A5]
Protein G purified
(applications tested for the base form of this product only)
Specificity and Use
Siglec-5-Fc protein, consisting of the full-length extracellular region of human Siglec-5, fused with the Fc region of human IgG1.
Clone 1A5 recognizes human Siglec-5 (Sialic acid-binding Ig-like lectin 5), otherwise known as CD170, a novel sialic-acid-binding Ig-like lectin, and member of the Ig superfamily, expressed by dendritic cells (DCs), activated macrophages, neutrophils, and cells of the monocyte/myeloid lineage. Clone 1A5, is one of several Siglec-5 antibodies which also recognizes human Siglec-14 (Sialic acid-binding Ig-like lectin 14) see for details. Siglec-14 shares an almost identical sequence with Siglec-5 within the first two Ig-like domains, indicating partial gene conversion between these two Siglecs, also evident in other primate species. Siglec-5 is highly related to the myelomonocytic-derived adhesion molecule CD33 (Siglec-3), and mediates sialic-acid dependent binding to cells, as well as acting as an inhibitory receptor in the down-regulation of cell activation. Structurally, Siglec-5 contains an immunoreceptor tyrosine-based inhibitor motif (ITIM), which plays a part in the modulation of cellular responses, and when phosphorylated, can bind to the SH2 domain of several SH2-containing phosphatases. Siglec-14 is a putative sialic-acid binding adhesion molecule, and member of the Ig superfamily, predominantly expressed in hematopoietic tissues, which has been shown to associate with the activating adapter protein DAP12. Clone 1A5 has been show to cross react with Chimpanzee see for details.
The applications listed have been tested for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.
PBS, 0.09% sodium azide, 1% BSA
+4°C or -20°C, Avoid repeated freezing and thawing.
Human peripheral blood monocytes stained with Mouse anti-Human Siglec-5/Siglec-14 followed by rabbit F(ab')2 anti-mouse IgG:FITC. This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.
Human peripheral blood monocytes stained with Mouse anti-Human Siglec-5/Siglec-14:FITC
Requested From: United States
Date Requested: 10/22/2016