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Anti-SIGLEC5 + SIGLEC14 Antibody (clone 1A5, FITC) LS-C187567

Ordering

Wt. Vol. Conc. Price
100 µg - 0.1 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SIGLEC5 + SIGLEC14 Antibodies

Anti-SIGLEC5 + SIGLEC14 Antibody (clone 1A5, FITC) LS-C187567
Mouse Monoclonal [clone 1A5] (IgG1) to Human SIGLEC5 + SIGLEC14
Human
Immunofluorescence, Immunoprecipitation, Flow Cytometry, ELISA
FITC Conjugated
Flow Cytometry Image
Anti-SIGLEC5 + SIGLEC14 Antibody (clone 1A5) LS-C187568
Mouse Monoclonal [clone 1A5] (IgG1) to Human SIGLEC5 + SIGLEC14
Human
Immunofluorescence, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated
Flow Cytometry Image
Anti-SIGLEC5 + SIGLEC14 Antibody (aa91-140) LS-C387024
Rabbit Polyclonal (IgG) to Human SIGLEC5 + SIGLEC14
Human
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1A5] (IgG1) to Human SIGLEC5 + SIGLEC14
Human
Immunofluorescence, Immunoprecipitation, Flow Cytometry, ELISA (applications tested for the base form of this product only)
FITC Conjugated

Details

Human SIGLEC5 + SIGLEC14
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [1A5]
Fluorescein. Also available Unconjugated.
Protein G purified
Unmodified

Applications

  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
  • ELISA
  • (applications tested for the base form of this product only)

Specificity and Use

Siglec-5-Fc protein, consisting of the full-length extracellular region of human Siglec-5, fused with the Fc region of human IgG1.
Clone 1A5 recognizes human Siglec-5 (Sialic acid-binding Ig-like lectin 5), otherwise known as CD170, a novel sialic-acid-binding Ig-like lectin, and member of the Ig superfamily, expressed by dendritic cells (DCs), activated macrophages, neutrophils, and cells of the monocyte/myeloid lineage. Clone 1A5, is one of several Siglec-5 antibodies which also recognizes human Siglec-14 (Sialic acid-binding Ig-like lectin 14) see for details. Siglec-14 shares an almost identical sequence with Siglec-5 within the first two Ig-like domains, indicating partial gene conversion between these two Siglecs, also evident in other primate species. Siglec-5 is highly related to the myelomonocytic-derived adhesion molecule CD33 (Siglec-3), and mediates sialic-acid dependent binding to cells, as well as acting as an inhibitory receptor in the down-regulation of cell activation. Structurally, Siglec-5 contains an immunoreceptor tyrosine-based inhibitor motif (ITIM), which plays a part in the modulation of cellular responses, and when phosphorylated, can bind to the SH2 domain of several SH2-containing phosphatases. Siglec-14 is a putative sialic-acid binding adhesion molecule, and member of the Ig superfamily, predominantly expressed in hematopoietic tissues, which has been shown to associate with the activating adapter protein DAP12. Clone 1A5 has been show to cross react with Chimpanzee see for details.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, 0.09% sodium azide, 1% BSA
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About SIGLEC5 + SIGLEC14


Flow Cytometry

Flow Cytometry
Human peripheral blood monocytes stained with Mouse anti-Human Siglec-5/Siglec-14 followed by rabbit F(ab')2 anti-mouse IgG:FITC. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry
Human peripheral blood monocytes stained with Mouse anti-Human Siglec-5/Siglec-14:FITC This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/8/2016

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