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Anti-SGK1 / SGK Antibody (clone 4D7-G3) LS-C133430

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular SGK1 / SGK Antibodies

Anti-SGK1 / SGK Antibody (clone 3E3) IHC-plus™ LS-B5532
Mouse Monoclonal [clone 3E3] (IgG2b,k) to Human SGK1 / SGK
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SGK1 / SGK Antibody (clone 3G8) IHC-plus™ LS-B5535
Mouse Monoclonal [clone 3G8] (IgG1,k) to Human SGK1 / SGK
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-SGK1 / SGK Antibody (aa323-352, Biotin) LS-C269205
Rabbit Polyclonal (IgG) to Mouse SGK1 / SGK
Mouse, Human
Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 4D7-G3] (IgG1,k) to Human SGK1 / SGK
Human
Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human SGK1 / SGK
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [4D7-G3]
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

  • IHC - Paraffin

Specificity and Use

SGK1 / SGK antibody was raised against sGK (AAH01263, 1 a.a. ~ 432 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human SGK1
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About SGK1 / SGK

O00141 NM_005627 NP_005618.2

SGK1 Antibody, Hsgk Antibody, Hsgk1 Antibody, SGK Antibody, Sgk1 variant i3 Antibody

Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cellular enzymes, transcription factors, neuronal excitability, cell growth, proliferation, survival, migration and apoptosis. Plays an important role in cellular stress response.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to SGK on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot
Western blot of SGK expression in transfected 293T cell line by SGK monoclonal antibody (M01), clone 4D7-G3.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged SGK is approximately 1 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot of SGK over-expressed 293 cell line, cotransfected with SGK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SGK monoclonal antibody clone 4D7-G3. GAPDH ( 36.1 kD ) used as specificity and.

Requested From: United States
Date Requested: 12/8/2016

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