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Catalog Number Size Price
LS-B69-50 50 µg (1 mg/ml) $460 
SGK1 / SGK Antibody - Anti-SGK1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

IHC‑plus™ Polyclonal Rabbit anti‑Human SGK1 / SGK Antibody (aa400‑431, IHC, WB) LS‑B69

IHC‑plus™ Polyclonal Rabbit anti‑Human SGK1 / SGK Antibody (aa400‑431, IHC, WB) LS‑B69

Note: This antibody replaces LS-C18997
Antibody:
SGK1 / SGK Rabbit anti-Human Polyclonal (aa400-431) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B69-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
SGK1 / SGK Rabbit anti-Human Polyclonal (aa400-431) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
SGK antibody LS-B69 is an unconjugated rabbit polyclonal antibody to SGK (SGK1) (aa400-431) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human SGK1 / SGK
Synonyms
SGK1 | Hsgk | Hsgk1 | SGK | Sgk1 variant i3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Mouse, Rat, Bovine, Dog, Rabbit, Chicken, Macaque (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
SKG-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 400-431 of human SGK -1.
Epitope
aa400-431
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human, macaque and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with SGK-1 homologues from mouse, rat, dog, chicken, bovine, rabbit and spiny dogfish based on an 92% sequence homology for the immunogen (12/13) from these sources. Cross reactivity with SGK-1 homologues from other sources has not been determined.
Applications
  • IHC
  • IHC - Paraffin (10 - 20 µg/ml)
  • Western blot (1:500 - 1:3000)
  • ELISA (1:4000 - 1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B69 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B69 was determined to be 10-20 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SGK1 / SGK
O00141 NM_005627 NP_005618.2

Validation

SGK1 / SGK Antibody - Anti-SGK1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-SGK1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-SGK1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

LSBio Ratings

IHC-plus™ SGK1 / SGK Antibody (aa400-431) for IHC, WB/Western, ELISA LS-B69 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022