Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone OX-85] (IgG1) to Rat SELL / L-Selectin / CD62L
Flow Cytometry (applications tested for the base form of this product only)
Rat SELL / L-Selectin / CD62L
Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [OX-85]
Also available conjugated with FITC, RPE.
Flow Cytometry (1:1 - 1:10)
(applications tested for the base form of this product only)
Specificity and Use
Recognizes rat L-selectin (CD62L). In rats CD62L is expressed by a subset of thymocytes, predominantly single positive CD4 or CD8 cells. Expression of rat CD62L is weak, and sensitive detection systems (e. g. 3 layer biotin - streptavidin techniques) are recommended on some tissues.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
PBS, pH 7.4, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
SELL / L-Selectin / CD62L is a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites.