Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Human SELL / L-Selectin / CD62L
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Rat Monoclonal [clone MEL-14] (IgG2a,k) to Mouse SELL / L-Selectin / CD62L
Mouse SELL / L-Selectin / CD62L
Mouse (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [MEL-14]
Specificity and Use
The MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.06 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide
SELL / L-Selectin / CD62L is a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites.
Staining of C57Bl/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.03 ug APC anti-mouse CD62L (MEL-14) (colored histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.