Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal to Human SELE / CD62E / E-selectin
Human, Monkey, Mouse, Rat
IHC, IHC - Paraffin, Western blot
Mouse Monoclonal [clone P2H3] (IgG1) to Human SELE / CD62E / E-selectin
Immunoprecipitation, Flow Cytometry
Human SELE / CD62E / E-selectin
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [P2H3]
IHC - Paraffin
Specificity and Use
This P2H3 antibody has been tested by flow cytometric analysis of TNFα-actiavted Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
SELE / CD62E / E-selectin is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules.
Staining of TNFalpha-activated Human Umbilical Vein Endothelial Cells (HUVEC) with 0.5 ug of Purified Mouse IgG1, K isotype control (open histogram) or 0.5 ug of Purified anti-human CD62E (P2H3) (colored histogram) followed by PE Donkey F(ab')2 Fragment Anti-Mouse IgG (H+L, Minimal Reactivity to Rat IgG). Total viable cells were used for analysis.