TMEM66 antibody LS-C81004 is an unconjugated rabbit polyclonal antibody to TMEM66 (SARAF) from human, mouse, rat and other species. Validated for WB.
Human SARAF / TMEM66
Human, Monkey, Mouse, Rat, Guinea pig
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
- Western blot
- (applications tested for the base form of this product only)
SARAF / TMEM66 antibody was raised against synthetic peptide located between aa218-267 of human TMEM66 (Q96BY9, NP_057211). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat (100%); Rabbit (92%); Elephant, Guinea pig (85%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C81004. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.