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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RPS6KB1 / P70S6K / S6K Antibody (clone 6B2) LS‑C172782
S6K antibody LS-C172782 is an unconjugated mouse monoclonal antibody to S6K (RPS6KB1 / P70S6K) from human, mouse, dog and other species. Validated for IF, IHC and WB.
Catalog
Size
Price
LS-C172782-100
100 µl
Unavailable

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Product Description

S6K antibody LS-C172782 is an unconjugated mouse monoclonal antibody to S6K (RPS6KB1 / P70S6K) from human, mouse, dog and other species. Validated for IF, IHC and WB.
About RPS6KB1 / P70S6K / S6K
Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. P23443 NM_003161 NP_003152.1

RPS6KB1 Antibody, 70 kDa S6 kinase Antibody, p70 S6KA Antibody, p70-alpha Antibody, p70-S6K Antibody, p70 S6 kinase alpha Antibody, p70s6 kinase Antibody, S6K-beta-1 Antibody, STK14A Antibody, p70 ribosomal S6 kinase Antibody, p70 S6 kinase, alpha 1 Antibody, p70 S6 kinase, alpha 2 Antibody, p70 S6K-alpha Antibody, p70-S6K 1 Antibody, p70S6K Antibody, p70S6K1 Antibody, PS6K Antibody, Ribosomal protein S6 kinase I Antibody, S6K Antibody, S6K1 Antibody, p70 ribosomal S6 kinase alpha Antibody, p70(S6K)-alpha Antibody


Specifications

Target
Human RPS6KB1 / P70S6K / S6K
Host
Mouse
Reactivity
Human, Monkey, Mouse, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [6B2]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Immunofluorescence (1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length human recombinant protein of human RPS6KB1(NP_003152) produced in HEK293T cell.
Specificity
Human P70S6K / S6K
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Human tonsil using anti-RPS6KB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human tonsil using anti-RPS6KB1 mouse monoclonal antibody.

Immunofluorescence

Anti-RPS6KB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPS6KB1.
Anti-RPS6KB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPS6KB1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPS6KB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPS6KB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPS6KB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPS6KB1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-RPS6KB1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-RPS6KB1 monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human tonsil using anti-RPS6KB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human tonsil using anti-RPS6KB1 mouse monoclonal antibody.

Immunofluorescence

Anti-RPS6KB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPS6KB1.
Anti-RPS6KB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPS6KB1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPS6KB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPS6KB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPS6KB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPS6KB1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-RPS6KB1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-RPS6KB1 monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human tonsil using anti-RPS6KB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human tonsil using anti-RPS6KB1 mouse monoclonal antibody.

Immunofluorescence

Anti-RPS6KB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPS6KB1.
Anti-RPS6KB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPS6KB1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPS6KB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPS6KB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPS6KB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPS6KB1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-RPS6KB1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-RPS6KB1 monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human tonsil using anti-RPS6KB1 mouse monoclonal antibody.
IHC of paraffin-embedded Human tonsil using anti-RPS6KB1 mouse monoclonal antibody.

Immunofluorescence

Anti-RPS6KB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPS6KB1.
Anti-RPS6KB1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPS6KB1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPS6KB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPS6KB1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPS6KB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPS6KB1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-RPS6KB1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-RPS6KB1 monoclonal antibody.


Requested From: 
Date Requested: 6/18/2018

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