Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Human RPS6KB1 / P70S6K / S6K
Human, Monkey, Mouse, Rat
Western blot, Immunoprecipitation, ELISA
Human RPS6KB1 / P70S6K / S6K
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Protein A purified
Western blot (1:1000)
Specificity and Use
RPS6KB1 / P70S6K / S6K antibody was raised against synthetic peptide derived from the sequence near the C terminus of human p70 S6 kinase (KLH).
Recognizes total levels of endogenous human p70 S6 kinase protein at 70kD. Also recognizes p85 S6 kinase at 85kD. Species cross-reactivity: mouse, rat and monkey.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000, Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50.
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex.