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Anti-RPA2 / RFA2 / RPA34 Antibody (clone 7C12) LS-C114862

Ordering

Wt. Vol. Conc. Price
- 100 µl - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RPA2 / RFA2 / RPA34 Antibodies

Anti-RPA2 / RFA2 / RPA34 Antibody LS-C38952
Rat Monoclonal to Human RPA2 / RFA2 / RPA34
Human, Monkey, Mouse, Rat, Hamster
ICC, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Anti-RPA2 / RFA2 / RPA34 Antibody (phospho-Ser4) LS-C286650
Rabbit Polyclonal (IgG) to Human RPA2 / RFA2 / RPA34
Human, Mouse
IHC - Paraffin, Immunofluorescence
Unconjugated
Immunohistochemistry Image
Anti-RPA2 / RFA2 / RPA34 Antibody (phospho-Ser4/Ser8) LS-C287160
Rabbit Polyclonal (IgG) to Human RPA2 / RFA2 / RPA34
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Proximity Ligation Assay
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 7C12] (IgG2a) to Human RPA2 / RFA2 / RPA34
Human, Monkey, Dog
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human RPA2 / RFA2 / RPA34
Mouse
Human, Monkey, Dog (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [7C12]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation
  • Flow Cytometry (1:100)

Specificity and Use

RPA2 / RFA2 / RPA34 antibody was raised against full length human recombinant protein of human RPA2 (NP_002937) produced in HEK293T cell.
Human RPA2 / RPA34
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About RPA2 / RFA2 / RPA34

P15927 NM_002946 NP_002937.1

RPA2 Antibody, RF-A protein 2 Antibody, RP-A p34 Antibody, RPA34 Antibody, Replication protein A2, 32kDa Antibody, RP-A p32 Antibody, REPA2 Antibody, Replication factor A protein 2 Antibody, Replication protein A2 (32kD) Antibody, RPA32 Antibody

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Ovary tissue using anti-RPA2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Kidney tissue using anti-RPA2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded pancreas tissue using anti-RPA2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded prostate tissue using anti-RPA2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded endometrium tissue using anti-RPA2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-RPA2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded breast tissue using anti-RPA2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-RPA2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded colon tissue using anti-RPA2 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Immunofluorescence
Anti-RPA2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2.

Immunofluorescence

Immunofluorescence
Immunofluorescent staining of HepG2 cells using anti-RPA2 mouse monoclonal antibody.

Western blot

Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-RPA2 monoclonal antibody.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.

Flow Cytometry

Flow Cytometry
Flow cytometry of HeLa cells, using anti-RPA2 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow Cytometry
Flow cytometry of HeLa cells, using anti-RPA2 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either pCMV6-ENTRY RPA2 (Red) or empty vector control plasmid (Blue) were immunostained with anti-RPA2 mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.

Requested From: United States
Date Requested: 12/2/2016

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