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Anti-RPA2 / RFA2 / RPA34 Antibody (clone 10G1) LS-C114840

Ordering

Wt. Vol. Conc. Price
- 100 µl - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RPA2 / RFA2 / RPA34 Antibodies

Anti-RPA2 / RFA2 / RPA34 Antibody LS-C38952
Rat Monoclonal to Human RPA2 / RFA2 / RPA34
Human, Monkey, Mouse, Rat, Hamster
ICC, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Anti-RPA2 / RFA2 / RPA34 Antibody (phospho-Ser4) LS-C286650
Rabbit Polyclonal (IgG) to Human RPA2 / RFA2 / RPA34
Human, Mouse
IHC - Paraffin, Immunofluorescence
Unconjugated
Immunohistochemistry Image
Anti-RPA2 / RFA2 / RPA34 Antibody (phospho-Ser4/Ser8) LS-C287160
Rabbit Polyclonal (IgG) to Human RPA2 / RFA2 / RPA34
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Proximity Ligation Assay
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 10G1] (IgG1) to Human RPA2 / RFA2 / RPA34
Human
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human RPA2 / RFA2 / RPA34
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [10G1]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Western blot (1:4000)
  • Immunoprecipitation
  • Flow Cytometry (1:100)

Specificity and Use

RPA2 / RFA2 / RPA34 antibody was raised against full length human recombinant protein of human RPA2 (NP_002937) produced in HEK293T cell.
Human RPA2 / RPA34
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About RPA2 / RFA2 / RPA34

P15927 NM_002946 NP_002937.1

RPA2 Antibody, RF-A protein 2 Antibody, RP-A p34 Antibody, RPA34 Antibody, Replication protein A2, 32kDa Antibody, RP-A p32 Antibody, REPA2 Antibody, Replication factor A protein 2 Antibody, Replication protein A2 (32kD) Antibody, RPA32 Antibody

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded liver tissue using anti-RPA2 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Ovary tissue using anti-RPA2 mouse monoclonal antibody. (Dilution 1:50).

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RPA2 antibody, and then analyzed by flow cytometry.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either pCMV6-ENTRY RPA2 (Red) or empty vector control plasmid (Blue) were immunostained with anti-RPA2 mouse monoclonal(Dilution 1:1,000), and then analyzed by flow cytometry.
 

Requested From: United States
Date Requested: 12/4/2016

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