Target
Human ROCK2
Synonyms
ROCK2 | KIAA0619 | p164 ROCK-2 | Rho kinase 2 | RhoA-associated kinase-2 | ROCK-II | Rho kinase II | Rok beta | Rok2 | RockII
Reactivity
Human
(tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Bovine, Dog, Chicken, Xenopus, Drosophila (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y256 of human ROCK-2.
Specificity
This antibody is specific for human ROCK-2 protein phosphorylated at Y256. A BLAST analysis was used to suggest cross-reactivity with ROCK-2 from human, mouse, rat, chicken, dog, bovine, Xenopus and Drosophila based on a 100% homology with the immunizing sequence. Cross-reactivity with ROCK-2 from other sources has not been determined.
Applications
- IHC
- Western blot (1:200 - 1:2000)
- ELISA (1:2000 - 1:20000)
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Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 164 kD in size corresponding to phosphorylated ROCK-2 by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human ROCK-2 pY256 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee