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Anti-RNF25 Antibody (C-Terminus) IHC-plus™ LS-B418

Note: This antibody replaces LS-C19052


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)

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Rabbit Polyclonal to Human RNF25
Human, Mouse
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA


Human RNF25
Human, Mouse (tested or 100% immunogen sequence identity)
Immunoaffinity purified


IHC - Paraffin (5 µg/ml), Western blot (1:200 - 1:2000), Immunoprecipitation, ELISA (1:10000 - 1:40000)

Specificity and Use

RNF25 antibody was raised against synthetic peptide from human RNF25.
Amino acids near the carboxyl terminus of human RNF25 protein.
Immunohistochemistry: LS-B418 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B418 was determined to be 5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About RNF25

Q96BH1 NM_022453 NP_071898.2

RNF25 Antibody, AO7 Antibody, Ring finger protein 25 Antibody, Ring finger protein AO7 Antibody

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). Stimulates transcription mediated by NF-kappa-B.


Anti-RNF25 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B418 concentration 5 ug/ml.

Western blot

Western blot
Anti-RNF25 Antibody - Immunoprecipitation. 1 ug of affinity purified anti-RNF25 was used in immunoprecipitation of 20 ug HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti-HA tag antibody. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.

Western blot

Western blot
Anti-RNF25 Antibody - Western Blot. Western blot of affinity purified anti-RNF25 antibody shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 1 is a vector only control. The extracts were loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:1000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.

Requested From: United States
Date Requested: 12/1/2015

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