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Anti-RNF216 / TRIAD3 Antibody (aa350-400) IHC-plus™ LS-B2090

Note: This antibody replaces LS-C834, LS-C89685
Catalog Size Price
LS-B2090-50 50 µg (0.5 mg/ml) Unavailable

Most Popular RNF216 / TRIAD3 Antibodies

Anti-RNF216 / TRIAD3 Antibody (N-Terminus) IHC-plus™ LS-B1430
Rabbit Polyclonal to Mouse RNF216 / TRIAD3
Mouse, Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-RNF216 / TRIAD3 Antibody (aa350-400) IHC-plus™ LS-B2090
Rabbit Polyclonal (IgG) to Human RNF216 / TRIAD3
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-RNF216 / TRIAD3 Antibody (aa716-745) LS-C98008
Rabbit Polyclonal to Human RNF216 / TRIAD3
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-RNF216 / TRIAD3 Antibody (C-Terminus, AP) LS-C246208
Rabbit Polyclonal (IgG) to Human RNF216 / TRIAD3
Human, Mouse
IHC, Western blot, ELISA
AP Conjugated
Anti-RNF216 / TRIAD3 Antibody (C-Terminus) LS-C330709
Rabbit Polyclonal (IgG) to Human RNF216 / TRIAD3
Human, Mouse
IHC, Western blot, ELISA
Unconjugated

100% Guaranteed
Rabbit Polyclonal (IgG) to Human RNF216 / TRIAD3
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human RNF216 / TRIAD3
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)

Specificity and Use

RNF216 / TRIAD3 antibody was raised against synthetic peptide from human RNF216 / TRIAD3.
aa350-400
KLH-conjugated synthetic peptide corresponding to a portion of human TRIAD3 between amino acids 350-400.
Immunohistochemistry: LS-B2090 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2090 was determined to be 5 ug/ml.

Packaging

PBS, 0.2% gelatin, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About RNF216 / TRIAD3

Q9NWF9 NM_207111 NP_996994.1

RNF216 Antibody, TRIAD3 Antibody, UBCE7IP1 Antibody, Ring finger protein 216 Antibody, U7I1 Antibody, ZIN Antibody

This gene encodes a cytoplasmic protein which specifically colocalizes and interacts with the serine/threonine protein kinase, receptor-interacting protein (RIP). Zinc finger domains of the encoded protein are required for its interaction with RIP and for inhibition of TNF- and IL1-induced NF-kappa B activation pathways. The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes and transfers it to substrates.

Immunohistochemistry

Immunohistochemistry
Anti-RNF216 / TRIAD3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2090 concentration 5 ug/ml.

Western blot

Western blot
Western blot of TRIAD3 in human (A)Brain; (B) Heart; (C) Small Intestine; (D) Kidney; (E) Liver; (F) Lung; (G) Skeletal Muscle; (H) Pancreas; (I) Spleen; (J) Ovary; (K) Testis using antibody at 1 ug/ml.

Requested From: 
Date Requested: 6/25/2017

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