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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RASSF1 / RASSF1A Antibody (aa201‑250) LS‑C135582
RASSF1A antibody LS-C135582 is an unconjugated rabbit polyclonal antibody to RASSF1A (RASSF1) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C135582-50
50 µg
Unavailable

Popular RASSF1 / RASSF1A Products

Antibody
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
A431 cells were labeled with anti-RASSF1a(clone 3F3) mAb, and detection was using a biotinylated secondary antibody and Texas-red conj streptavidin.
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Anti-RASSF1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3784 concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Anti-RASSF1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4170 concentration 3.75 ug/ml.
Species: Human, Bat, Bovine, Horse, Pig
Applications: IHC, IHC - Paraffin
Anti-RASSF1 / RASSF1A antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10044 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

RASSF1A antibody LS-C135582 is an unconjugated rabbit polyclonal antibody to RASSF1A (RASSF1) from human, mouse, rat and other species. Validated for WB.
About RASSF1 / RASSF1A
Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Q9NS23 NM_007182 NP_009113.3

RASSF1 Antibody, 123F2 Antibody, RASSF1A Antibody, RDA32 Antibody, Tumor suppressor protein RDA32 Antibody, NORE2A Antibody, WUGSC:H_LUCA12.5 Antibody, REH3P21 Antibody


Specifications

Target
Human RASSF1 / RASSF1A
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Sheep, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Monkey, Guinea pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa201-250 of human RASSF1 (Q9NS23, NP_733831). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Hamster, Elephant, Dog, Bovine, Bat, Horse, Pig (100%); Marmoset, Rabbit, Guinea pig (92%); Platypus, Lizard, Xenopus (85%); Panda, Opossum, Turkey, Zebra finch, Chicken, Stickleback (84%).
Blocking Peptide
LS-E21832 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135582. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa201-250
Specificity
Human RASSF1 / RASSF1A
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

RASSF1 / RASSF1A antibody LS-C135582 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RASSF1 / RASSF1A antibody LS-C135582 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RASSF1 / RASSF1A antibody LS-C135582 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RASSF1 / RASSF1A antibody LS-C135582 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RASSF1 / RASSF1A antibody LS-C135582 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RASSF1 / RASSF1A antibody LS-C135582 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

RASSF1 / RASSF1A antibody LS-C135582 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
RASSF1 / RASSF1A antibody LS-C135582 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

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