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RASSF1 / RASSF1A Antibody (aa201‑250) LS‑C135582
RASSF1A antibody LS-C135582 is an unconjugated rabbit polyclonal antibody to RASSF1A (RASSF1) from human, mouse, rat and other species. Validated for WB.
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Review This ProductPopular RASSF1 / RASSF1A Products
Species:
Human
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human, Bat, Bovine, Horse, Pig
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Product Description
RASSF1A antibody LS-C135582 is an unconjugated rabbit polyclonal antibody to RASSF1A (RASSF1) from human, mouse, rat and other species. Validated for WB.
About RASSF1 / RASSF1A
Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression.
Q9NS23
NM_007182
NP_009113.3
RASSF1 Antibody, 123F2 Antibody, RASSF1A Antibody, RDA32 Antibody, Tumor suppressor protein RDA32 Antibody, NORE2A Antibody, WUGSC:H_LUCA12.5 Antibody, REH3P21 Antibody
Specifications
Target
Human RASSF1 / RASSF1A
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Sheep, Zebrafish
(tested or 100% immunogen sequence identity)
Predicted
Monkey, Guinea pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot (1 µg/ml)
- (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa201-250 of human RASSF1 (Q9NS23, NP_733831). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Hamster, Elephant, Dog, Bovine, Bat, Horse, Pig (100%); Marmoset, Rabbit, Guinea pig (92%); Platypus, Lizard, Xenopus (85%); Panda, Opossum, Turkey, Zebra finch, Chicken, Stickleback (84%).
Blocking Peptide
LS-E21832 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135582. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C135582. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa201-250
Specificity
Human RASSF1 / RASSF1A
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Western blot
RASSF1 / RASSF1A antibody LS-C135582 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
RASSF1 / RASSF1A antibody LS-C135582 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
RASSF1 / RASSF1A antibody LS-C135582 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
RASSF1 / RASSF1A antibody LS-C135582 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/18/2018
Date Requested: 6/18/2018
