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Anti-RASSF1 / RASSF1A Antibody (aa201-250) LS-C135582

Catalog	Size	Price
LS-C135582-50	50 µg	Unavailable

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Anti-RASSF1 / RASSF1A Antibody (aa135-147) IHC-plus™ LS-B4170

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Reactivity:	Human, Bat, Bovine, Horse, Pig
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Anti-RASSF1 / RASSF1A Antibody (clone 8E4) IHC-plus™ LS-B10044

Antibody:	Mouse Monoclonal [clone 8E4] (IgG1) to Human RASSF1 / RASSF1A
Reactivity:	Human
Application:	IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
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Antibody:	Rabbit Polyclonal to Human RASSF1 / RASSF1A
Reactivity:	Human, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Sheep, Zebrafish
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Human RASSF1 / RASSF1A
Host:	Rabbit
Reactivity:	Human, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Sheep, Zebrafish (tested or 100% immunogen sequence identity)
Predicted:	Monkey, Guinea pig, Rabbit (at least 90% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

Synthetic peptide located between aa201-250 of human RASSF1 (Q9NS23, NP_733831). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Sheep, Hamster, Elephant, Dog, Bovine, Bat, Horse, Pig (100%); Marmoset, Rabbit, Guinea pig (92%); Platypus, Lizard, Xenopus (85%); Panda, Opossum, Turkey, Zebra finch, Chicken, Stickleback (84%).

Blocking Peptide:

LS-E21832 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135582. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa201-250

Specificity:

Human RASSF1 / RASSF1A

Usage:

Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About RASSF1 / RASSF1A

Uniprot: Q9NS23 NCBI: NM_007182 NP_009113.3

Synonyms: RASSF1 Antibody, 123F2 Antibody, RASSF1A Antibody, RDA32 Antibody, Tumor suppressor protein RDA32 Antibody, NORE2A Antibody, WUGSC:H_LUCA12.5 Antibody, REH3P21 Antibody

Description: Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression.

(More About RASSF1 / RASSF1A)

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Western blot

RASSF1 / RASSF1A antibody LS-C135582 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 8/17/2017

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