Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
RAN Antibody, Gsp1 Antibody, Guanosine triphosphatase Ran Antibody, RanGTPase Antibody, Member RAS oncogene family Antibody, OK/SW-cl.81 Antibody, Ras-related nuclear protein Antibody, ARA24 Antibody, TC4 Antibody, GTPase Ran Antibody, Ras-like protein TC4 Antibody
RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis.
Formalin-fixed and paraffin-embedded human brain tissue reacted with RAN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of RAN (arrow) using rabbit polyclonal RAN Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAN gene.
Western blot of RAN Antibody in HL-60 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.
Western blot of RAN antibody in NIH-3T3 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.
RAN Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.