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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RAN Antibody (aa12‑39) LS‑C162428
RAN antibody LS-C162428 is an unconjugated rabbit polyclonal antibody to RAN from human and mouse. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C162428-400
400 µl
Unavailable

Popular RAN Products

Antibody
Species: Human, Monkey, Mouse, Rat, Hamster, Drosophila
Applications: Western blot
RAN antibody LS-C107810 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish, Drosophila
Applications: Western blot
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Anti-RAN antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8294 dilution 3.75 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Pig, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA

Product Description

RAN antibody LS-C162428 is an unconjugated rabbit polyclonal antibody to RAN from human and mouse. Validated for Flow, IHC and WB.
About RAN
RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. P62826 NM_006325 NP_006316.1

RAN Antibody, Gsp1 Antibody, Guanosine triphosphatase Ran Antibody, RanGTPase Antibody, Member RAS oncogene family Antibody, OK/SW-cl.81 Antibody, Ras-related nuclear protein Antibody, ARA24 Antibody, TC4 Antibody, GTPase Ran Antibody, Ras-like protein TC4 Antibody


Specifications

Target
Human RAN
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Monkey, Rat, Chicken, Xenopus, Zebrafish, Yeast, C. elegans, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8389 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C162428. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa12-39
Specificity
This RAN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human RAN.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with RAN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with RAN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of RAN (arrow) using rabbit polyclonal RAN Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAN gene.
Western blot of RAN (arrow) using rabbit polyclonal RAN Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAN gene.

Western blot

Western blot of RAN Antibody in HL-60 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.
Western blot of RAN Antibody in HL-60 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.

Western blot

Western blot of RAN antibody in NIH-3T3 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.
Western blot of RAN antibody in NIH-3T3 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.

Flow Cytometry

RAN Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
RAN Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with RAN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with RAN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of RAN (arrow) using rabbit polyclonal RAN Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAN gene.
Western blot of RAN (arrow) using rabbit polyclonal RAN Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAN gene.

Western blot

Western blot of RAN Antibody in HL-60 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.
Western blot of RAN Antibody in HL-60 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.

Western blot

Western blot of RAN antibody in NIH-3T3 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.
Western blot of RAN antibody in NIH-3T3 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.

Flow Cytometry

RAN Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
RAN Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with RAN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with RAN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of RAN (arrow) using rabbit polyclonal RAN Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAN gene.
Western blot of RAN (arrow) using rabbit polyclonal RAN Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAN gene.

Western blot

Western blot of RAN Antibody in HL-60 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.
Western blot of RAN Antibody in HL-60 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.

Western blot

Western blot of RAN antibody in NIH-3T3 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.
Western blot of RAN antibody in NIH-3T3 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.

Flow Cytometry

RAN Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
RAN Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with RAN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with RAN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of RAN (arrow) using rabbit polyclonal RAN Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAN gene.
Western blot of RAN (arrow) using rabbit polyclonal RAN Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RAN gene.

Western blot

Western blot of RAN Antibody in HL-60 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.
Western blot of RAN Antibody in HL-60 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.

Western blot

Western blot of RAN antibody in NIH-3T3 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.
Western blot of RAN antibody in NIH-3T3 cell line lysates (35 ug/lane). RAN (arrow) was detected using the purified antibody.

Flow Cytometry

RAN Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
RAN Antibody flow cytometry of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/19/2018

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