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Anti-RAMA1 / SKA3 Antibody (aa311-323) IHC-plus™ LS-B7373

Note: This antibody replaces LS-C112839

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RAMA1 / SKA3 Antibodies

Anti-RAMA1 / SKA3 Antibody (C-Terminus) IHC-plus™ LS-B5039
Rabbit Polyclonal to Human RAMA1 / SKA3
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-RAMA1 / SKA3 Antibody (aa311-323) IHC-plus™ LS-B7373
Goat Polyclonal to Human RAMA1 / SKA3
Human, Dog
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-RAMA1 / SKA3 Antibody (Internal, AP) LS-C243973
Rabbit Polyclonal (IgG) to Human RAMA1 / SKA3
Human
Western blot, ELISA
AP Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human RAMA1 / SKA3
Human, Dog
IHC - Paraffin, ELISA
Unconjugated

Details

Human RAMA1 / SKA3
Goat
Human, Dog (tested or 100% immunogen sequence identity)
Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (3.75 µg/ml)
  • ELISA (1:64000)

Specificity and Use

RAMA1 / SKA3 antibody was raised against synthetic peptide C-PLSKTNSSSNDLE from an internal region of human SKA3 (NP_659498.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Dog (100%); Panda, Rabbit (92%); Monkey, Marmoset, Mouse, Toxoplasma (85%).
LS-E27158 - Lyophilized - 100 µg - $145.00
aa311-323
Human SKA3.
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 35kDa and 150kDa band in HeLa lysates after 2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 46.4kDa according to NP_659498.3. Both bands were successfully blocked by incubation with the immunizing peptide.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About RAMA1 / SKA3

Q8IX90 NM_145061 NP_659498.4

SKA3 Antibody, C13orf3 Antibody, RAMA1 Antibody

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization.

Immunohistochemistry

Immunohistochemistry
Anti-SKA3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7373 concentration 3.75 ug/ml.
 

Requested From: United States
Date Requested: 12/8/2016

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