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Anti-RAD50 Antibody (aa628-787) IHC-plus™ LS-B302

Note: This antibody replaces LS-C2860
Catalog Size Price
LS-B302-100 100 µl Unavailable

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100% Guaranteed
Rabbit Polyclonal to Human RAD50
Human
IHC - Paraffin
Unconjugated

Details

Human RAD50
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

IHC - Paraffin (1:500)

Specificity and Use

RAD50 antibody was raised against human Rad50 zinc hook region (amino acids 628-787) expressed in E. coli
aa628-787
Human Rad50.
Immunohistochemistry: LS-B302 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B302 was determined to be 1:500.

Packaging

Whole antisera
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About RAD50

Q92878 NM_005732 NP_005723.2

RAD50 Antibody, DNA repair protein RAD50 Antibody, HRad50 Antibody, NBSLD Antibody, RAD50 (S. cerevisiae) homolog Antibody, RAD50 homolog (S. cerevisiae) Antibody, RAD502 Antibody, RAD50-2 Antibody

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity.

Immunohistochemistry

Immunohistochemistry
Anti-RAD50 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B302 dilution 1:500.

Requested From: 
Date Requested: 6/27/2017

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