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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RAD50 Antibody (aa628‑787) IHC‑plus™ LS‑B302 Best Seller
Note: This antibody replaces LS-C2860
RAD50 antibody LS-B302 is an unconjugated rabbit polyclonal antibody to human RAD50. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B302-100
100 µl
Unavailable

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Product Description

RAD50 antibody LS-B302 is an unconjugated rabbit polyclonal antibody to human RAD50. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
About RAD50
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. Q92878 NM_005732 NP_005723.2

RAD50 Antibody, DNA repair protein RAD50 Antibody, HRad50 Antibody, NBSLD Antibody, RAD50 (S. cerevisiae) homolog Antibody, RAD50 homolog (S. cerevisiae) Antibody, RAD502 Antibody, RAD50-2 Antibody


Specifications

Target
Human RAD50
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RAD50 antibody was raised against human Rad50 zinc hook region (amino acids 628-787) expressed in E. coli
Epitope
aa628-787
Specificity
Human Rad50.
Usage
Immunohistochemistry: LS-B302 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B302 was determined to be 1:500.
Presentation
Whole antisera
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-RAD50 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B302 dilution 1:500.
Anti-RAD50 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B302 dilution 1:500.

Immunohistochemistry

Anti-RAD50 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B302 dilution 1:500.
Anti-RAD50 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B302 dilution 1:500.

Immunohistochemistry

Anti-RAD50 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B302 dilution 1:500.
Anti-RAD50 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B302 dilution 1:500.

Immunohistochemistry

Anti-RAD50 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B302 dilution 1:500.
Anti-RAD50 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B302 dilution 1:500.


Requested From: 
Date Requested: 6/23/2018

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