(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:500)
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RAD50 antibody was raised against human Rad50 zinc hook region (amino acids 628-787) expressed in E. coli
Immunohistochemistry: LS-B302 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B302 was determined to be 1:500.
4°C or -20°C, Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.