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RAD50

RAD50 homolog (S. cerevisiae)

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

Gene Name: RAD50 homolog (S. cerevisiae)
Synonyms: RAD50, DNA repair protein RAD50, HRad50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2
Target Sequences: NM_005732 NP_005723.2 Q92878

Publications (25)

1
Human MRE11 is inactivated in mismatch repair-deficient cancers. Giannini G, Ristori E, Cerignoli F, Rinaldi C, Zani M, Viel A, Ottini L, Crescenzi M, Martinotti S, Bignami M, Frati L, Screpanti I, Gulino A. EMBO reports. 2002 3:248-54. [PubMed:11850399] [PMC:PMC1084012]
2
Phosphorylation of histone H2AX and activation of Mre11, Rad50, and Nbs1 in response to replication-dependent DNA double-strand breaks induced by mammalian DNA topoisomerase I cleavage complexes. Furuta T, Takemura H, Liao ZY, Aune GJ, Redon C, Sedelnikova OA, Pilch DR, Rogakou EP, Celeste A, Chen HT, Nussenzweig A, Aladjem MI, Bonner WM, Pommier Y. The Journal of biological chemistry. 2003 278:20303-12. (WB; Human) [PubMed:12660252]
3
Telomere maintenance and cell cycle regulation in spontaneously immortalized T-cell lines from Nijmegen breakage syndrome patients. Siwicki JK, Degerman S, Chrzanowska KH, Roos G. Experimental cell research. 2003 287:178-89. (WB, IP, Flo; Human) [PubMed:12799193]
4
Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation. Udayakumar D, Bladen CL, Hudson FZ, Dynan WS. The Journal of biological chemistry. 2003 278:41631-5. (WB; Human) [PubMed:12917393]
5
Senescing human cells and ageing mice accumulate DNA lesions with unrepairable double-strand breaks. Sedelnikova OA, Horikawa I, Zimonjic DB, Popescu NC, Bonner WM, Barrett JC. Nature cell biology. 2004 6:168-70. (Mouse) [PubMed:14755273]
6
Linkage between Werner syndrome protein and the Mre11 complex via Nbs1. Cheng WH, von Kobbe C, Opresko PL, Arthur LM, Komatsu K, Seidman MM, Carney JP, Bohr VA. The Journal of biological chemistry. 2004 279:21169-76. (ICC, WB, IP; Human) [PubMed:15026416]
7
Independent roles for nibrin and Mre11-Rad50 in the activation and function of Atm. Cerosaletti K, Concannon P. The Journal of biological chemistry. 2004 279:38813-9. (ICC, WB; Human) [PubMed:15234984]
8
Defective DNA strand break repair after DNA damage in prostate cancer cells: implications for genetic instability and prostate cancer progression. Fan R, Kumaravel TS, Jalali F, Marrano P, Squire JA, Bristow RG. Cancer research. 2004 64:8526-33. [PubMed:15574758]
9
Mdm2 binds to Nbs1 at sites of DNA damage and regulates double strand break repair. Alt JR, Bouska A, Fernandez MR, Cerny RL, Xiao H, Eischen CM. The Journal of biological chemistry. 2005 280:18771-81. (ICC, WB; Human) [PubMed:15734743]
10
Active role for nibrin in the kinetics of atm activation. Cerosaletti K, Wright J, Concannon P. Molecular and cellular biology. 2006 26:1691-9. [PubMed:16478990] [PMC:PMC1430256]
11
The Rb-related p130 protein controls telomere lengthening through an interaction with a Rad50-interacting protein, RINT-1. Kong LJ, Meloni AR, Nevins JR. Molecular cell. 2006 22:63-71. [PubMed:16600870]
12
Mre11-Rad50-Nbs1 complex is activated by hypertonicity. Sheen MR, Kim SW, Jung JY, Ahn JY, Rhee JG, Kwon HM, Woo SK. American journal of physiology. Renal physiology. 2006 291:F1014-20. [PubMed:16788144]
13
Defective Mre11-dependent activation of Chk2 by ataxia telangiectasia mutated in colorectal carcinoma cells in response to replication-dependent DNA double strand breaks. Takemura H, Rao VA, Sordet O, Furuta T, Miao ZH, Meng L, Zhang H, Pommier Y. The Journal of biological chemistry. 2006 281:30814-23. [PubMed:16905549]
14
Dimerization of the Rad50 protein is independent of the conserved hook domain. Cahill D, Carney JP. Mutagenesis. 2007 22:269-74. [PubMed:17426050]
15
DNA double-strand breaks form in bystander cells after microbeam irradiation of three-dimensional human tissue models. Sedelnikova OA, Nakamura A, Kovalchuk O, Koturbash I, Mitchell SA, Marino SA, Brenner DJ, Bonner WM. Cancer research. 2007 67:4295-302. [PubMed:17483342]
16
RIDDLE immunodeficiency syndrome is linked to defects in 53BP1-mediated DNA damage signaling. Stewart GS, Stankovic T, Byrd PJ, Wechsler T, Miller ES, Huissoon A, Drayson MT, West SC, Elledge SJ, Taylor AM. Proceedings of the National Academy of Sciences of the United States of America. 2007 104:16910-5. [PubMed:17940005] [PMC:PMC2040433]
17
Chronic hypoxia decreases synthesis of homologous recombination proteins to offset chemoresistance and radioresistance. Chan N, Koritzinsky M, Zhao H, Bindra R, Glazer PM, Powell S, Belmaaza A, Wouters B, Bristow RG. Cancer research. 2008 68:605-14. [PubMed:18199558]
18
A glycine-arginine domain in control of the human MRE11 DNA repair protein. Dry U, Coulombe Y, Rodrigue A, Stasiak A, Richard S, Masson JY. Molecular and cellular biology. 2008 28:3058-69. [PubMed:18285453] [PMC:PMC2293076]
19
Mdm2 promotes genetic instability and transformation independent of p53. Bouska A, Lushnikova T, Plaza S, Eischen CM. Molecular and cellular biology. 2008 28:4862-74. [PubMed:18541670] [PMC:PMC2493369]
20
Temporal regulation of the Mre11-Rad50-Nbs1 complex during adenovirus infection. Karen KA, Hoey PJ, Young CS, Hearing P. Journal of virology. 2009 83:4565-73. [PubMed:19244322] [PMC:PMC2668508]
21
Rad50 zinc hook is important for the Mre11 complex to bind chromosomal DNA double-stranded breaks and initiate various DNA damage responses. He J, Shi LZ, Truong LN, Lu CS, Razavian N, Li Y, Negrete A, Shiloach J, Berns MW, Wu X. The Journal of biological chemistry. 2012 287:31747-56. (WB; Human) [PubMed:22833675] [PMC:PMC3442509]
22
The Mre11 nuclease is critical for the sensitivity of cells to Chk1 inhibition. Thompson R, Montano R, Eastman A. PloS one. 2012 7:e44021. (WB; Human) [PubMed:22937147] [PMC:PMC3427249]
23
PALB2 self-interaction controls homologous recombination. Buisson R, Masson JY. Nucleic acids research. 2012 40:10312-23. (Human) [PubMed:22941656] [PMC:PMC3488246]
24
The RING finger protein RNF8 ubiquitinates Nbs1 to promote DNA double-strand break repair by homologous recombination. Lu CS, Truong LN, Aslanian A, Shi LZ, Li Y, Hwang PY, Koh KH, Hunter T, Yates JR, Berns MW, Wu X. The Journal of biological chemistry. 2012 287:43984-94. (WB, IP; Human) [PubMed:23115235] [PMC:PMC3527981]
25
Mitochondrial dysfunction in a novel form of autosomal recessive ataxia. Murad NA, Cullen JK, McKenzie M, Ryan MT, Thorburn D, Gueven N, Kobayashi J, Birrell G, Yang J, Drk T, Becherel O, Grattan-Smith P, Lavin MF. Mitochondrion. 2013 13:235-45. (WB; Human) [PubMed:23178371]
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Filtered By: None
Target
RAD50 (75)
Reactivity
Human (55)
Mouse (36)
Rat (13)
Bat (4)
Bovine (7)
Chicken (1)
Chimpanzee (3)
Dog (6)
Gibbon (3)
Guinea pig (4)
Hamster (1)
Horse (5)
Monkey (5)
Orangutan (3)
Rabbit (7)
Rodent (1)
Yeast (1)
Application
IHC (36)
IHC-Fr (1)
IHC-P (5)
WB (72)
Flo (1)
ELISA (20)
ICC (3)
IF (4)
IP (20)
Peptide-ELISA (1)
PLA (1)
Host
rabbit (59)
mouse (14)
sheep (2)
Isotype
IgG (48)
IgG1 (4)
IgG1,k (8)
IgG2a (1)
IgG2b,k (1)
Clonality
monoclonal mc (14)
polyclonal pc (61)
Clone
13B3 (1)
3D2G10 (1)
4H9 (1)
5A8E5 (1)
AB01/1A7 (1)
GF1 (1)
Format
AP Conjugated (2)
APC (3)
APC Conjugated (5)
Azide-free (1)
Biotin Conjugated (7)
Cy3 Conjugated (3)
Cy7 Conjugated (3)
FITC Conjugated (8)
HRP Conjugated (6)
PE Conjugated (5)
Unconjugated (36)
Epitope
aa1-234 (10)
aa400-595 (9)
aa631-793 (8)
aa911-938 (8)
N-Terminus (5)
aa1-425 (4)
C-Terminus (2)
Internal (2)
aa480-724 (2)
aa628-787 (2)
aa700-750 (2)
650-730 aa, Internal (1)
Ala708 (1)
aa50-100 (1)
aa681-730 (1)
Publications
No (75)
Showing 1-25 of 75 results

RAD50 Antibody (aa1-425, clone 13B3) IHC-plus™ LS-B1708
Mouse Monoclonal [clone 13B3] (IgG1) to Human RAD50
Human
IF IHC IHC-P IP WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-RAD50 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-RAD50 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

RAD50 Antibody (aa628-787) IHC-plus™ LS-B302
Rabbit Polyclonal to Human RAD50
Human
IHC IHC-P
Unconjugated
100µl  $425.00
Anti-RAD50 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-RAD50 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

RAD50 Antibody (aa700-750) IHC-plus™ LS-B12399
Rabbit Polyclonal (IgG) to Human RAD50
Rabbit, Mouse, Dog, Bovine, Human
IF IHC IHC-P
Unconjugated
50µl  $425.00
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

RAD50 Antibody (aa628-787) LS-C93574
Rabbit Polyclonal to Human RAD50
Human
IP WB
Unconjugated
100µl  $515.00

RAD50 Antibody LS-C332354
Rabbit Polyclonal (IgG) to Human RAD50
Mouse, Rat, Human
IF IHC IP WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded human gastric cancer tissue.
Immunohistochemistry of paraffin-embedded human metrocarcinoma tissue.
Immunohistochemistry of paraffin-embedded human gastric cancer tissue.
Immunohistochemistry of paraffin-embedded human metrocarcinoma tissue.

RAD50 Antibody LS-C17558
Rabbit Polyclonal (IgG) to Human RAD50
Rodent, Human
ELISA ICC IHC IHC-Fr WB
Unconjugated
50µg  $375.00

RAD50 Antibody (aa700-750) LS-C287108
Rabbit Polyclonal (IgG) to Human RAD50
Rabbit, Dog, Bovine, Human
IF IHC IHC-P IP WB
Unconjugated
100µl 1mg/ml  $395.00
Detection of Human Rad50 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Rad50 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Detection of Human Rad50 by Immunoprecipitation and Western Blot. Sample: Nuclear extract (50 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-Rad50 used at 0.25 ug/ml. Detection: Chemiluminescence.
Detection of Human Rad50 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Rad50 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Detection of Human Rad50 by Immunoprecipitation and Western Blot. Sample: Nuclear extract (50 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-Rad50 used at 0.25 ug/ml. Detection: Chemiluminescence.

RAD50 Antibody LS-C402529
Rabbit Polyclonal (IgG) to Human RAD50
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl 0.4mg/ml  $295.00; 200µl  $395.00
Immunohistochemistry of Human colon cancer using RAD50 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human gastric cancer using RAD50 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human colon cancer using RAD50 Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of Human gastric cancer using RAD50 Polyclonal Antibody at dilution of 1:40.

RAD50 Antibody (Internal) LS-C353235
Rabbit Polyclonal to Human RAD50
Chicken, Mouse, Rat, Human
IHC IHC-P IP WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of RAD50 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of RAD50 expression in HeLa (A); K562 (B); Jurkat (C); MCF7 (D); Ramos (E); SW480 (F) whole cell lysates.
Immunohistochemical analysis of RAD50 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of RAD50 expression in HeLa (A); K562 (B); Jurkat (C); MCF7 (D); Ramos (E); SW480 (F) whole cell lysates.

RAD50 Antibody LS-C746738
Rabbit Polyclonal (IgG) to Human RAD50
Mouse, Human
IHC WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Immunohistochemistry of paraffin-embedded human uterine cancer using RAD50 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RAD50 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using RAD50 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RAD50 Antibody at dilution of 1:200 (40x lens).

RAD50 Antibody (650-730 aa, Internal) LS-C385731
Rabbit Polyclonal (IgG) to Human RAD50
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of Rad50 antibody
Western blot of Rad50 antibody

RAD50 Antibody (aa1-234) LS-C373714
Rabbit Polyclonal (IgG) to Mouse RAD50
Mouse
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $545.00; 10ml 0.5mg/ml  $2,160.00
Western blot of RAD50 antibody.
Western blot of RAD50 antibody.

RAD50 Antibody (aa1-234, Biotin) LS-C424868
Rabbit Polyclonal (IgG) to Mouse RAD50
Mouse
ICC WB
Biotin Conjugated
100µl 0.5mg/ml  $375.00

RAD50 Antibody (aa1-234, FITC) LS-C424870
Rabbit Polyclonal (IgG) to Mouse RAD50
Mouse
ICC WB
FITC Conjugated
100µl 0.5mg/ml  $395.00

RAD50 Antibody (aa1-234, APC, Cy7) LS-C737269
Rabbit Polyclonal (IgG) to Mouse RAD50
Mouse
IHC WB
APC, Cy7 Conjugated
20µl  $285.00; 100µl  $465.00; 200µl  $650.00; 1ml  $1,620.00; 10ml  $6,480.00
Western blot of RAD50 antibody.
Western blot of RAD50 antibody.

RAD50 Antibody (clone 3D2G10) LS-C669432
Mouse Monoclonal [clone 3D2G10] (IgG2a) to Human RAD50
Human
ELISA Flo
Unconjugated
0.1mg  $365.00

RAD50 Antibody (aa911-938) LS-C324503
Rabbit Polyclonal (IgG) to Mouse RAD50
Mouse
ELISA WB
Unconjugated
200µl  $530.00

RAD50 Antibody (Ala708) LS-C192003
Rabbit Polyclonal to Human RAD50
Mouse, Rat, Human
IHC WB
Unconjugated
100µl  $345.00

RAD50 Antibody LS-C341294
Rabbit Polyclonal to Human RAD50
Mouse, Human
IHC WB
Unconjugated
100µl 1mg/ml  $385.00

RAD50 Antibody (aa631-793) LS-C293708
Rabbit Polyclonal (IgG) to Human RAD50
Human
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $530.00; 10ml 0.5mg/ml  $2,100.00
Western blot of recombinant RAD50.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant RAD50.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RAD50 Antibody (aa400-595) LS-C293710
Rabbit Polyclonal (IgG) to Human RAD50
Human
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $530.00; 10ml 0.5mg/ml  $2,100.00
Western blot of recombinant RAD50.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant RAD50.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

RAD50 Antibody (clone 5A8E5) LS-C669428
Mouse Monoclonal [clone 5A8E5] (IgG1) to Human RAD50
Human
ELISA WB
Unconjugated
0.1mg  $365.00

RAD50 Antibody (FITC) LS-C538984
Mouse Monoclonal (IgG1,k) to Human RAD50
Human
ELISA IP WB
FITC Conjugated
100µl  $815.00

RAD50 Antibody (PE) LS-C657132
Mouse Monoclonal (IgG1,k) to Human RAD50
Human
ELISA IP WB
PE Conjugated
100µl  $815.00

RAD50 Antibody (AP) LS-C510968
Mouse Monoclonal (IgG1,k) to Human RAD50
Human
ELISA IP WB
AP Conjugated
100µl  $815.00
Showing 1-25 of 75 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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