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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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RAD21 Antibody (C‑Terminus, clone CM110‑2C10, DY550) LS‑C141130
RAD21 antibody LS-C141130 is a DY550-conjugated mouse monoclonal antibody to RAD21 from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C141130-100
100 µl
Unavailable

Popular RAD21 Products

Anti-RAD21 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B82 concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Anti-RAD21 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemical analysis of RAD21 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine, Zebrafish
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA

Product Description

RAD21 antibody LS-C141130 is a DY550-conjugated mouse monoclonal antibody to RAD21 from human and mouse. Validated for IHC and WB.
About RAD21
Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. O60216 NM_006265 NP_006256.1

RAD21 Antibody, CDLS4 Antibody, HR21 Antibody, KIAA0078 Antibody, HHR21 Antibody, NXP-1 Antibody, NXP1 Antibody, RAD21 (S. pombe) homolog Antibody, MCD1 Antibody, Nuclear matrix protein 1 Antibody, RAD21 homolog (S. pombe) Antibody, HRAD21 Antibody, SCC1 Antibody, SCC1 homolog Antibody


Specifications

Target
Human RAD21
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [CM110-2C10]
Conjugations
DyLight 550. Also available Unconjugated or conjugated with Biotin, DY650, DY488.
Purification
Purified IgG
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:500)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RAD21 antibody was raised against an N-terminal fusion protein of the C-terminal fragment of Scc1 subunit human cohesin (amino acids 544-631 of hRad21)
Epitope
C-Terminus
Specificity
both the nuclear hRad21 protein and the cleaved cytoplasmic hRad21 protein.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM sodium borate
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-RAD21 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B82 concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-RAD21 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B82 concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot with monoclonal anti-Rad21 in HEK293 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot with monoclonal anti-Rad21 in HEK293 lysates. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Cohesin detected in HeLa cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Cohesin detected in HeLa cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-RAD21 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B82 concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-RAD21 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B82 concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot with monoclonal anti-Rad21 in HEK293 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot with monoclonal anti-Rad21 in HEK293 lysates. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Cohesin detected in HeLa cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Cohesin detected in HeLa cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-RAD21 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B82 concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-RAD21 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B82 concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot with monoclonal anti-Rad21 in HEK293 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot with monoclonal anti-Rad21 in HEK293 lysates. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Cohesin detected in HeLa cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Cohesin detected in HeLa cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-RAD21 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B82 concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-RAD21 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B82 concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot with monoclonal anti-Rad21 in HEK293 lysates.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot with monoclonal anti-Rad21 in HEK293 lysates. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Cohesin detected in HeLa cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Cohesin detected in HeLa cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

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