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Anti-RAD18 Antibody (aa1-259) LS-C186340

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.46 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular RAD18 Antibodies

Anti-RAD18 Antibody (aa332-431, APC) LS-C226215
Mouse Monoclonal (IgG2b) to Human RAD18
Human
IHC, ICC, Western blot, ELISA
APC Conjugated
Anti-RAD18 Antibody (aa332-431, Biotin) LS-C226216
Mouse Monoclonal (IgG2b) to Human RAD18
Human
IHC, ICC, Western blot, ELISA
Biotin Conjugated
Anti-RAD18 Antibody (aa332-431, PE) LS-C226219
Mouse Monoclonal (IgG2b) to Human RAD18
Human
IHC, ICC, Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human RAD18
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human RAD18
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

RAD18 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 259 of RAD18 (SwissProt Q9NS91). Percent identity by BLAST analysis: Human (100%); Bovine (80%).
aa1-259
Human RAD18
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About RAD18

NM_020165

RAD18 Antibody, HHR18 Antibody, HRAD18 Antibody, RNF73 Antibody, RAD18 homolog (S. cerevisiae) Antibody, RAD18, S. cerevisiae, homolog Antibody, RING finger protein 73 Antibody

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Cal27 Xenograft using RAD18 antibody at 1:100 dilution.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. RAD18 antibody diluted at 1:1000.

Requested From: United States
Date Requested: 12/3/2016

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