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Anti-PUF60 Antibody (aa371-420) LS-C80370

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PUF60 Antibodies

Anti-PUF60 Antibody (aa371-420) LS-C80370
Rabbit Polyclonal (IgG) to Human PUF60
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PUF60 Antibody (C-Terminus) IHC-plus™ LS-B12600
Rabbit Polyclonal (IgG) to Human PUF60
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human PUF60
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human PUF60
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse (tested or 100% immunogen sequence identity)
Bat (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Protein A purified
Unmodified

Applications

  • IHC - Paraffin
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

PUF60 antibody was raised against synthetic peptide located between aa371-420 of human PUF60 (Q9UHX1-2, NP_055096). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Opossum, Guinea pig (100%); Orangutan, Bat (92%); Turkey, Zebra finch, Chicken, Xenopus (85%).
LS-E14937 - Lyophilized - 100 µg - $145.00
aa371-420
Human PUF60
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
100 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About PUF60

Q9UHX1 NM_014281 Q9UHX1

PUF60 Antibody, FBP-interacting repressor Antibody, FBP interacting repressor Antibody, RoBPI Antibody, Siah-BP1 Antibody, Ro-binding protein 1 Antibody, RoBP1 Antibody, Siah binding protein 1 Antibody, FIR Antibody, Siah-binding protein 1 Antibody, SIAHBP1 Antibody

DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity.

Immunohistochemistry

Immunohistochemistry
PUF60 antibody LS-C80370 was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Transfected 293T cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/7/2016

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