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Anti-PSMD10 / Gankyrin Antibody (aa100-200) IHC-plus™ LS-B300

Note: This antibody replaces LS-C2786, LS-C89606

Ordering

Wt. Vol. Conc. Price
- 50 µl 0.97 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PSMD10 / Gankyrin Antibodies

Anti-PSMD10 / Gankyrin Antibody (aa100-200) IHC-plus™ LS-B300
Rabbit Polyclonal to Human PSMD10 / Gankyrin
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PSMD10 / Gankyrin Antibody (aa184-213, FITC) LS-C207539
Rabbit Polyclonal (IgG) to Human PSMD10 / Gankyrin
Human
Western blot, ELISA
FITC Conjugated
Anti-PSMD10 / Gankyrin Antibody (clone 4B5) IHC-plus™ LS-B10019
Mouse Monoclonal [clone 4B5] (IgG1,k) to Human PSMD10 / Gankyrin
Human
IHC - Frozen, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human PSMD10 / Gankyrin
Human, Mouse
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human PSMD10 / Gankyrin
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (2 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

PSMD10 / Gankyrin antibody was raised against a synthetic peptide made to an internal portion of the human gankyrin protein sequence (between residues 100-200). UniProt O75832. Percent identity by BLAST analysis: Mouse (100%).
aa100-200
Gankyrin.
Immunohistochemistry: LS-B300 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B300 was determined to be 5 ug/ml.

Packaging

Buffer, 0.05% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About PSMD10 / Gankyrin

O75832 NM_002814 NP_002805.1

PSMD10 Antibody, 26s proteasome subunit 10 Antibody, Ankyrin repeat protein Antibody, DJ889N15.2 Antibody, Gankyrin Antibody, p28 Antibody, p28(GANK) Antibody

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pahway, leading to prolonged AKT activation.

Immunohistochemistry

Immunohistochemistry
Anti-PSMD10 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B300 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/9/2016

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