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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PSMC3 Antibody (clone 1C10) LS‑C114986
PSMC3 antibody LS-C114986 is an unconjugated mouse monoclonal antibody to PSMC3 from human, rat, dog and other species. Validated for IF, IHC and WB.
Catalog
Size
Price
LS-C114986-100
100 µl
Unavailable

Popular PSMC3 Products

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Western blot of extracts from K562/HT-29 cells, using PRS6A Antibody. The lane on the right is treated with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
PSMC3 monoclonal antibody (M01), clone 1B9 Western Blot analysis of PSMC3 expression in HeLa.
Species: Human
Applications: Western blot, ELISA
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

PSMC3 antibody LS-C114986 is an unconjugated mouse monoclonal antibody to PSMC3 from human, rat, dog and other species. Validated for IF, IHC and WB.
About PSMC3
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. P17980 NM_002804 NP_002795.2

PSMC3 Antibody, Proteasome subunit P50 Antibody, TBP1 Antibody, TBP-1 Antibody, Rpt5 Antibody, Tat-binding protein 1 Antibody


Specifications

Target
Human PSMC3
Host
Mouse
Reactivity
Human, Monkey, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [1C10]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PSMC3 antibody was raised against full length human recombinant protein of human PSMC3(NP_002795) produced in HEK293T cell.
Specificity
Human TBP-1 / Rpt5
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded colon tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-PSMC3 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMC3.
Anti-PSMC3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMC3.

Immunofluorescence

Immunofluorescent staining of HepG2 cells using anti-PSMC3 mouse monoclonal antibody.
Immunofluorescent staining of HepG2 cells using anti-PSMC3 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMC3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMC3.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PSMC3 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PSMC3 monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded colon tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-PSMC3 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMC3.
Anti-PSMC3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMC3.

Immunofluorescence

Immunofluorescent staining of HepG2 cells using anti-PSMC3 mouse monoclonal antibody.
Immunofluorescent staining of HepG2 cells using anti-PSMC3 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMC3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMC3.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PSMC3 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PSMC3 monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded colon tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-PSMC3 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMC3.
Anti-PSMC3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMC3.

Immunofluorescence

Immunofluorescent staining of HepG2 cells using anti-PSMC3 mouse monoclonal antibody.
Immunofluorescent staining of HepG2 cells using anti-PSMC3 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMC3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMC3.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PSMC3 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PSMC3 monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Kidney tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Kidney tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded colon tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-PSMC3 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-PSMC3 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMC3.
Anti-PSMC3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMC3.

Immunofluorescence

Immunofluorescent staining of HepG2 cells using anti-PSMC3 mouse monoclonal antibody.
Immunofluorescent staining of HepG2 cells using anti-PSMC3 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMC3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMC3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMC3.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PSMC3 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PSMC3 monoclonal antibody.


Requested From: 
Date Requested: 6/20/2018

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