(tested or 100% immunogen sequence identity)
- Western blot (1 - 2 µg/ml)
- Immunoprecipitation (25 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Prss34 antibody was raised against nS0-derived recombinant mouse MCP-11/Prss34.
Recognizes mouse MCP-11/Prss34.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect mouse MCP-11. The detection limit for rmMCP-11 is approximately 10 ng/well. Western Blot: 1-2 ug/ml with the appropriate secondary reagents to detect mouse MCP-11. Using a colorimetric detection system, the detection limit for rmMCP-11 is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Immunoprecipitation: 25 ug/ml to immunoprecipitate rmMCP-11 from conditioned cell culture medium. The recovered rmMCP-11 can be detected in Western blots using a polyclonal antibody.
Lyophilized from PBS, 5% Trehalose
100 µl PBS, 40-50% glycerol
For research use only.