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Anti-Prohibitin 2 / PHB2 Antibody (aa88-299) IHC-plus™ LS-B2316

Note: This antibody replaces LS-C17619

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Prohibitin 2 / PHB2 Antibodies

Anti-Prohibitin 2 / PHB2 Antibody (C-Terminus) IHC-plus™ LS-B1889
Rabbit Polyclonal (IgG) to Mouse Prohibitin 2 / PHB2
Mouse, Human
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-Prohibitin 2 / PHB2 Antibody (aa1-50) LS-C90864
Rabbit Polyclonal to Human Prohibitin 2 / PHB2
Human
Immunoprecipitation
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human Prohibitin 2 / PHB2
Human, Rat
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated

Details

Human Prohibitin 2 / PHB2
Rabbit
Human, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation

Specificity and Use

Prohibitin 2 / PHB2 antibody was raised against his6-tagged fusion protein corresponding to aa88-299 of human REA-1. The immunizing sequence is identical in mouse.
aa88-299
Recognizes mouse REA, Mr 37kD. Species cross-reactivity: human and rat.
Immunohistochemistry: LS-B2316 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2316 was determined to be 5 ug/ml. Immunocytochemistry. Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: Immunoprecipitates REA from MCF-7 and 3T3 whole cell extracts. Immunocytochemistry: Nuclear staining of REA in paraformaldehyde fixed, Triton X-100 permeabilized MCF-7 cells.

Packaging

Tris-glycine, 0.15 M sodium chloride, pH 7.4, 0.05% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About Prohibitin 2 / PHB2

Q99623 NM_007273 NP_001138303.1

PHB2 Antibody, BCAP37 Antibody, BAP Antibody, D-prohibitin Antibody, p22 Antibody, Prohibitin-2 Antibody, B-cell associated protein Antibody, Bap37 Antibody, PNAS-141 Antibody, Prohibitin 2 Antibody, REA Antibody

Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases By similarity. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging.

Immunohistochemistry

Immunohistochemistry
Anti-PHB2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2316 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/8/2016

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