Target
Human PRKCB / PKC-Beta
Synonyms
PRKCB | PKC beta 2 | Pkc betaII | PRKCB2 | Protein kinase c beta | Protein kinase c beta 2 | Protein kinase c beta i | Protein kinase C beta type | Protein kinase C, beta | Protein kinase C, beta 1 | PRKCB1 | Pkc betaI | PKC-beta | PKCB | Pkcbeta | PkcbetaI | PKC-B | PkcbetaII | Protein kinase c beta 1 | Protein kinase c beta II
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Predicted
Monkey, Bovine, Rabbit (at least 90% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Protein kinase C beta.
Specificity
This antibody is specific for human PKC beta protein. A BLAST analysis was used to suggest cross-reactivity with PKC beta from human and monkey sources based on a 100% homology with the immunizing sequence. Expect partial cross reactivity to PKC beta from rabbit and bovine (93% homology) and rat and mouse (87% homology) sources. Cross-reactivity with PKC beta from other sources has not been determined.
Applications
- Western blot (1:1000 - 1:2000)
- ELISA (1:75000 - 1:80000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 76 kD in size corresponding to PKC beta by western blotting in the appropriate cell lysate or extract. Lysates from U251or BxPC3 cells are suggested for western blotting.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee