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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PRAME Antibody LS‑C47362 Best Seller
PRAME antibody LS-C47362 is an unconjugated rabbit polyclonal antibody to PRAME from human, bovine, dog and other species. Validated for WB.
Catalog
Size
Price
LS-C47362-100
100 µl
Unavailable

Popular PRAME Products

PRAME antibody LS-C111765 Western blot of Fetal Brain lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: Western blot
MAPE Antibody (RB18763) IHC of formalin-fixed and paraffin-embedded human testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Anti-PRAME antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10827 concentration 7.5 ug/ml.
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Immunofluorescence analysis of U2OS cells.
Species: Human
Applications: IHC, Immunofluorescence, Western blot

Product Description

PRAME antibody LS-C47362 is an unconjugated rabbit polyclonal antibody to PRAME from human, bovine, dog and other species. Validated for WB.
About PRAME
PRAME encodes a 509 amino acid antigen, lacking a signal sequence, and recognized on a human melanoma cell line by a T-lymphocyte clone. A significant level of PRAME mRNA is detected in normal testis as well as in many melanomas, non-small cell lung carcinomas, sarcomas, head and neck tumors and renal carcinomas. PRAME is expressed predominantly in acute leukemias carrying chromosomal abnormalities such as translocation t(8:21), which fuses the AML1 and ETO genes. P78395 NM_006115 NP_006106.1

PRAME Antibody, Cancer/testis antigen 130 Antibody, CT130 Antibody, MAPE Antibody, Opa-interacting protein 4 Antibody, Opa-interacting protein OIP4 Antibody, OIP-4 Antibody, OIP4 Antibody


Specifications

Target
Human PRAME
Host
Rabbit
Reactivity
Human, Monkey, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:5000)
Failed Applications
  • IHC - Paraffin
Immunogen
PRAME antibody was raised against a portion of amino acid 460-510 of human PRAME.
Specificity
A portion of amino acid 460-510 of human PRAME.
Presentation
Sera, 50% glycerol, 0.025% sodium azide.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of PRAME in MCF7 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.
Western blot of PRAME in MCF7 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.

Western blot

Western blot of PRAME in MCF7 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.
Western blot of PRAME in MCF7 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.

Western blot

Western blot of PRAME in MCF7 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.
Western blot of PRAME in MCF7 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.

Western blot

Western blot of PRAME in MCF7 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.
Western blot of PRAME in MCF7 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.


Requested From: 
Date Requested: 6/20/2018

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