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Anti-PPP1R14A / CPI-17 Antibody (aa1-147, clone 4H10) LS-C93787

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $315
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PPP1R14A / CPI-17 Antibodies

Anti-PPP1R14A / CPI-17 Antibody LS-C14630
Rabbit Polyclonal (IgG) to Pig PPP1R14A / CPI-17
Pig, Human, Mouse, Rat, Bovine, Rabbit
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 4H10] (IgG2a) to Human PPP1R14A / CPI-17
Human
Western blot, ELISA
Unconjugated

Details

Human PPP1R14A / CPI-17
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [4H10]
Unconjugated
Protein G purified
Unmodified

Applications

  • Western blot (1:250 - 1:1000)
  • ELISA

  • IHC - Paraffin

Specificity and Use

PPP1R14A / CPI-17 antibody was raised against recombinant human PPP1R14A (1-147aa) purified from E. coli.
aa1-147
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250-1:1000.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About PPP1R14A / CPI-17

Q96A00 NP_150281.1

PPP1R14A Antibody, 17-KDa protein Antibody, CPI-17 Antibody, PPP1INL Antibody, CPI17 Antibody

PPP1R14A / CPI-17 belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Western blot

Western blot
The Cell lysates of A549 (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1R14A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
 

Requested From: United States
Date Requested: 12/7/2016

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