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Anti-POU5F1B Antibody (aa214-242) IHC-plus™ LS-B10480

Note: This antibody replaces LS-C163329
Catalog Size Price
LS-B10480-20 20 µl Unavailable

Related Products

9 POU5F1B Antibodies

Most Popular POU5F1B Antibodies

Anti-POU5F1B Antibody (aa217-247, AP) LS-C265419
Rabbit Polyclonal (IgG) to Human POU5F1B
Human
Western blot, Flow Cytometry, ELISA
AP Conjugated
Anti-POU5F1B Antibody (aa217-247, APC) LS-C265420
Rabbit Polyclonal (IgG) to Human POU5F1B
Human
Western blot, Flow Cytometry, ELISA
APC Conjugated
Anti-POU5F1B Antibody (aa217-247, Biotin) LS-C265421
Rabbit Polyclonal (IgG) to Human POU5F1B
Human
Western blot, Flow Cytometry, ELISA
Biotin Conjugated
Anti-POU5F1B Antibody (aa214-242) IHC-plus™ LS-B10480
Rabbit Polyclonal to Human POU5F1B
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-POU5F1B Antibody (aa217-247) LS-C323744
Rabbit Polyclonal (IgG) to Human POU5F1B
Human
Western blot, Flow Cytometry, ELISA
Unconjugated

100% Guaranteed
Rabbit Polyclonal to Human POU5F1B
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human POU5F1B
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Pig, Cow (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

LS-E526 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B10480. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa214-242
This P5F1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-242 amino acids from the C-terminal region of human P5F1B.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About POU5F1B

Q06416 NP_001153014.1

POU5F1B Antibody, OCT4-PG1 Antibody, POU 5 domain protein Antibody, POU5F1P1 Antibody, POU5FLC20 Antibody, POU class 5 homeobox 1B Antibody, POU5F1P4 Antibody, POU5FLC8 Antibody, Octamer-binding protein 3-like Antibody, OTF3C Antibody, OTF3P1 Antibody

This intronless gene was thought to be a transcribed pseudogene of POU class 5 homeobox 1, however, it has been reported that this gene can encode a functional protein. The encoded protein is nearly the same length as and highly similar to the POU class 5 homeobox 1 transcription factor, has been shown to be a weak transcriptional activator and may play a role in carcinogenesis and eye development.

Immunohistochemistry

Immunohistochemistry
Anti-POU5F1B antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10480 dilution 1:100.

Western blot

Western blot
P5F1B Antibody western blot of K562 cell line lysates (35 ug/lane). The P5F1B antibody detected the P5F1B protein (arrow).

Flow Cytometry

Flow Cytometry
P5F1B Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 9/24/2017

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