Human (tested or 100% immunogen sequence identity)
Mouse, Bovine, Chicken, Xenopus (at least 90% immunogen sequence identity)
ICC (1:100 - 1:1000), Western blot (1:500 - 1:3000)
Specificity and Use
POLR3A antibody was raised against recombinant fragment corresponding to a region within amino acids 182 and 482 of POLR3A (SwissProt O14802). Percent identity by BLAST analysis: Human (100%); Bovine (98%); Mouse, Chicken (97%); Xenopus (94%).
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using POLR3A antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
Sample (30 ug of whole cell lysate). A: HCT116. 5% SDS PAGE. POLR3A antibody diluted at 1:500.
Requested From: United States
Date Requested: 4/18/2015