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Anti-POLK / DNA Polymerase Kappa Antibody (aa542-591) LS-C81298

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular POLK / DNA Polymerase Kappa Antibodies

Anti-POLK / DNA Polymerase Kappa Antibody (aa817-830) IHC-plus™ LS-B405
Goat Polyclonal to Human POLK / DNA Polymerase Kappa
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-POLK / DNA Polymerase Kappa Antibody (clone 6F2) IHC-plus™ LS-B5464
Mouse Monoclonal [clone 6F2] (IgG1,k) to Human POLK / DNA Polymerase Kappa
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-POLK / DNA Polymerase Kappa Antibody IHC-plus™ LS-B13397
Rabbit Polyclonal (IgG) to Human POLK / DNA Polymerase Kappa
Human, Mouse, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human POLK / DNA Polymerase Kappa
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human POLK / DNA Polymerase Kappa
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

POLK / DNA Polymerase Kappa antibody was raised against synthetic peptide located between aa542-591 of human POLK (Q9UBT6, NP_057302). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Horse, Pig (100%); Elephant (90%); Rabbit (85%).
LS-E17640 - Lyophilized - 100 µg - $145.00
aa542-591
Human POLK
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About POLK / DNA Polymerase Kappa

Q9UBT6 NM_016218 NP_057302.1

POLK Antibody, DINP Antibody, DNA polymerase kappa Antibody, DINB1 Antibody, DINB protein Antibody

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.

Western blot

Western blot
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/2/2016

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