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Anti-POLE3 / DNA Polymerase Epsilon Antibody (N-Terminus) LS-C30573

Catalog Size Price
LS-C30573-100 100 µg (1 mg/ml) Unavailable

Related Products

24 POLE3 / DNA Polymerase Epsilon Antibodies

Most Popular POLE3 / DNA Polymerase Epsilon Antibodies

Anti-POLE3 / DNA Polymerase Epsilon Antibody (aa97-147) LS-C286497
Rabbit Polyclonal (IgG) to Human POLE3 / DNA Polymerase Epsilon
Human, Mouse, Rat, Bovine, Guinea pig
IHC, IHC - Paraffin, Immunofluorescence
Unconjugated
Detection of Mouse PolE3/p17 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-PolE3/p17 used at a dilution of 1:100.
Anti-POLE3 / DNA Polymerase Epsilon Antibody (aa97-147) LS-C288056
Rabbit Polyclonal (IgG) to Human POLE3 / DNA Polymerase Epsilon
Human, Mouse, Rat, Bovine, Guinea pig
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Detection of Human and Mouse PolE3/p17 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PolE3/p17 used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
Anti-POLE3 / DNA Polymerase Epsilon Antibody LS-C335676
Rabbit Polyclonal (IgG) to Human POLE3 / DNA Polymerase Epsilon
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence analysis of U2OS cells.
Anti-POLE3 / DNA Polymerase Epsilon Antibody LS-C412380
Rabbit Polyclonal to Human POLE3 / DNA Polymerase Epsilon
Human, Mouse, Rat
Immunofluorescence, Western blot
Unconjugated
Anti-POLE3 / DNA Polymerase Epsilon Antibody LS-C482624
Rabbit Polyclonal to Human POLE3 / DNA Polymerase Epsilon
Human, Mouse, Rat
ICC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescent analysis of CHRAC17 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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Rabbit Polyclonal (IgG) to Human POLE3 / DNA Polymerase Epsilon
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated
Daudi Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Details

Human POLE3 / DNA Polymerase Epsilon
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Protein A purified
Unmodified

Applications

  • Western blot (2.5 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

Synthetic peptide from N-Terminus of human POLE3 (Q9NRF9, NP_059139). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus (100%); Frog (92%); Salmon, Stickleback, Zebrafish (84%).
LS-E11641 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C30573. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
N-Terminus
Human POLE3

Packaging

Lyophilized from PBS with 2% sucrose
100 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About POLE3 / DNA Polymerase Epsilon

Q9NRF9 NM_017443 NP_059139.3

POLE3 Antibody, CHRAC17 Antibody, CHRAC-17 Antibody, Histone fold protein CHRAC17 Antibody, HuCHRAC17 Antibody, p17 Antibody, YBL1 Antibody, Arsenic transactivated protein Antibody, Arsenic-transactivated protein Antibody, AsTP Antibody, CHARAC17 Antibody, DNA polymerase II subunit 3 Antibody

Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
(More About POLE3 / DNA Polymerase Epsilon)

Western blot

Daudi Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Daudi Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From: 
Date Requested: 4/23/2018
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