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POLE3 / DNA Polymerase Epsilon Antibody (N‑Terminus) LS‑C30573
DNA Polymerase Epsilon antibody LS-C30573 is an unconjugated rabbit polyclonal antibody to DNA Polymerase Epsilon (POLE3) from human, mouse, rat and other species. Validated for WB.
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Species:
Human, Mouse, Rat, Bovine, Guinea pig
Applications:
IHC, IHC - Paraffin, Immunofluorescence
Species:
Human, Mouse, Rat, Bovine, Guinea pig
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
ICC, Immunofluorescence, Western blot
Product Description
DNA Polymerase Epsilon antibody LS-C30573 is an unconjugated rabbit polyclonal antibody to DNA Polymerase Epsilon (POLE3) from human, mouse, rat and other species. Validated for WB.
About POLE3 / DNA Polymerase Epsilon
Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
Q9NRF9
NM_017443
NP_059139.3
POLE3 Antibody, CHRAC17 Antibody, CHRAC-17 Antibody, Histone fold protein CHRAC17 Antibody, HuCHRAC17 Antibody, p17 Antibody, YBL1 Antibody, Arsenic transactivated protein Antibody, Arsenic-transactivated protein Antibody, AsTP Antibody, CHARAC17 Antibody, DNA polymerase II subunit 3 Antibody
Specifications
Target
Human POLE3 / DNA Polymerase Epsilon
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Protein A purified
Modifications
Unmodified
Applications
- Western blot (2.5 µg/ml)
- (applications tested for the base form of this product only)
Immunogen
Synthetic peptide from N-Terminus of human POLE3 (Q9NRF9, NP_059139). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus (100%); Frog (92%); Salmon, Stickleback, Zebrafish (84%).
Blocking Peptide
LS-E11641 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C30573. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C30573. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human POLE3
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
100 µl After adding water, will consist of PBS buffer with 2% sucrose
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Western blot
Daudi Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Daudi Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Daudi Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Daudi Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/19/2018
Date Requested: 6/19/2018
