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| Catalog Number | Size | Price |
|---|---|---|
| LS-C153861-100 | 100 µg (1 mg/ml) | $502 |
Polyclonal Rabbit anti‑Human POLB / DNA Polymerase Beta Antibody (C‑Terminus, WB) LS‑C153861
Polyclonal Rabbit anti‑Human POLB / DNA Polymerase Beta Antibody (C‑Terminus, WB) LS‑C153861
Antibody:
POLB / DNA Polymerase Beta Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
POLB / DNA Polymerase Beta Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
DNA Polymerase Beta antibody LS-C153861 is an unconjugated rabbit polyclonal antibody to human DNA Polymerase Beta (POLB) (C-Terminus). Validated for ELISA and WB. Cited in 2 publications.
Target
Human POLB / DNA Polymerase Beta
Synonyms
POLB | DNA pol beta | Mutant DNA polymerase beta | DNA polymerase beta | DNA polymerase beta subunit
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Predicted
Bovine, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of the POLß protein.
Epitope
C-Terminus
Specificity
This antibody reacts with overexpressed and endogenous POLß protein. A BLAST analysis was used to suggest reactivity with POLß from human, bovine, and Xenopus laevis based on a 100% homology with the immunizing sequence. Cross-reactivity with POLß from other sources has not been determined.
Applications
- Western blot (0.5 µg/ml)
- ELISA (1:50000)
Usage
This affinity purified antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38.1 kD in size corresponding to POLbeta by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About POLB / DNA Polymerase Beta
LSBio Ratings
POLB / DNA Polymerase Beta Antibody (C-Terminus) for WB/Western, ELISA LS-C153861 has an LSBio Rating of
Publications (4.1)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Regulated over-expression of DNA polymerase beta mediates early onset cataract in mice. Sobol RW, Foley JF, Nyska A, Davidson MG, Wilson SH. DNA repair. 2003 2:609-22.
DNA polymerase beta-dependent long patch base excision repair in living cells. Asagoshi K, Liu Y, Masaoka A, Lan L, Prasad R, Horton JK, Brown AR, Wang XH, Bdour HM, Sobol RW, Taylor JS, Yasui A, Wilson SH. DNA repair. 2010 9:109-19.
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat, Bovine, Chicken
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
