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Anti-POLA1 / DNA Polymerase Alpha 1 Antibody (clone 3C11) LS-C197940

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular POLA1 / DNA Polymerase Alpha 1 Antibodies

Anti-POLA1 / DNA Polymerase Alpha 1 Antibody (Lys111) LS-C176726
Rabbit Polyclonal to Human POLA1 / DNA Polymerase Alpha 1
Human
Western blot
Unconjugated
Western blot Image
Anti-POLA1 / DNA Polymerase Alpha 1 Antibody (aa1363-1463, AP) LS-C226148
Mouse Monoclonal (IgG2a) to Human POLA1 / DNA Polymerase Alpha 1
Human
Western blot, ELISA
AP Conjugated
Anti-POLA1 / DNA Polymerase Alpha 1 Antibody (aa1363-1463, FITC) LS-C226151
Mouse Monoclonal (IgG2a) to Human POLA1 / DNA Polymerase Alpha 1
Human
Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 3C11] (IgG2a,k) to Human POLA1 / DNA Polymerase Alpha 1
Human
Western blot, ELISA
Unconjugated

Details

Human POLA1 / DNA Polymerase Alpha 1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [3C11]
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot
  • ELISA

Specificity and Use

POLA1 / DNA Polymerase Alpha 1 antibody was raised against pOLA (NP_058633, aa1363-1462 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human POLA1 / DNA Polymerase Alpha 1
Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About POLA1 / DNA Polymerase Alpha 1

P09884 NM_016937 NP_058633.2

POLA1 Antibody, p180 Antibody, POLA Antibody, DNA Polymerase Alpha 1 Antibody

Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands.

Western blot

Western blot
POLA monoclonal antibody (M01), clone 3C11 Western Blot analysis of POLA expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged POLA is approximately 0.3 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 12/8/2016

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