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Anti-PIM1 / Pim-1 Antibody (phospho-Tyr309) LS-C199440

Catalog Size Price
LS-C199440-50 50 µl Unavailable
LS-C199440-100 100 µl Unavailable

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100% Guaranteed
Rabbit Polyclonal (IgG) to Human PIM1 / Pim-1
Human, Mouse, Rat
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated

Details

Human PIM1 / Pim-1
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:40000)

Specificity and Use

PIM1 / Pim-1 antibody was raised against synthetic peptide derived from human Pim-1 around the phosphorylation site of Tyr309.
pTyr309
Pim-1 (Phospho-Tyr309) Antibody detects endogenous levels of Pim-1 only when phosphorylated at tyrosine309.

Packaging

PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Store at -20°C.
For research use only.

About PIM1 / Pim-1

P11309 NM_002648 NP_002639.1

PIM1 Antibody, Pim-1 kinase 44 kDa isoform Antibody, Proviral integration site 1 Antibody, Oncogene PIM1 Antibody, PIM Antibody, Pim-1 Antibody, Pim-1 oncogene Antibody

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity.

Western blot

Western blot of extracts from HUVEC cells treated with PMA 125ng/ml 30', using Pim-1 (Phospho-Tyr309) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HUVEC cells treated with PMA 125ng/ml 30', using Pim-1 (Phospho-Tyr309) Antibody. The lane on the right was incubated with synthetic peptide.

Requested From: 
Date Requested: 5/21/2018

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