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Anti-Phosphoserine Antibody LS-C74890

Catalog Size Price
LS-C74890-50 50 µg (0.25 mg/ml) Unavailable

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29 Phosphoserine Antibodies

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Anti-Phosphoserine Antibody LS-C74890
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Western blot analysis of the phosphorylated proteins with UV-treated cell lysates mouse spleen cell. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1 uM okadaic acid).

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Rabbit Polyclonal (IgG)
IHC, Western blot, Immunoprecipitation, ELISA (applications tested for the base form of this product only)


IgG Polyclonal
Unconjugated. Also available conjugated with HRP, HRP.
Immunoaffinity purified


  • IHC (1:50)
  • Western blot
  • Immunoprecipitation
  • ELISA (1:250 - 1:1000)
  • (applications tested for the base form of this product only)

Specificity and Use

Phosphoserine antibody was raised against phosphoserine conjugated with KLH.
Recognizes phosphoserine, peptidyl-phosphoserine and serine-phosphorylated proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein. Species cross-reactivity: All species.
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and ELISA. Western Blot (tissue lysates): 50-100 ug/lane total protein, antibody dilution can be from 1:250-1:1000, overloading will show solid exposed lanes. Dilute the primary antibody until multiple phosphorylated bands will appear. If using purified proteins dilutions from 1:50-1:1000 will need to be tried. At 1:300 dilution the antibody normally detects 10 ng of protein with 2 hour incubations and enhanced HRP detection. Blocking of the blot should be with non-phosphoserine containing compounds such as 1-5% BSA in TBS that has been filtered through a 0.45um. Milk or casein is not recommended because of the presence of phospho-serine in these solutions. Buffers should be Tris based so that phosphate ion interference is minimized. Immunoprecipitation (tissue extracts): 10-20 ug/mg protein. Note that this will immunoprecipitate all phosphoserine proteins in the extracts. If using purified protein exacts containing only the protein of interest, antibody amounts should be decreased to 5 ug/500 ul of extracts. ELISA (kinase assay): 1:250-1:500. Immunohistochemistry: 1:50. Detects all phosphoserines, and any phosphorylated serine protein or peptide can be used to block antibody staining. Typically, a10M excess of peptide is used in Tris based buffers. Positive control: NIH 3T3 cells (+/- TPA), K562 cells and EGF-stimulated A431 cells.


PBS, 50% glycerol.
For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.
For research use only.

About Phosphoserine

Requested From: 
Date Requested: 5/25/2018

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