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Anti-Phosphoserine Antibody LS-C74890

Catalog Size Price
LS-C74890-50 50 µg (0.25 mg/ml) Unavailable

Related Products

29 Phosphoserine Antibodies

Most Popular Phosphoserine Antibodies

Anti-Phosphoserine Antibody LS-C74890
Rabbit Polyclonal (IgG)
IHC, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-Phosphoserine Antibody LS-C74891
Mouse Monoclonal (IgG1)
Western blot, ELISA
Unconjugated
Anti-Phosphoserine Antibody LS-C74893
Rabbit Polyclonal (IgG)
IHC, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-Phosphoserine Antibody (clone 16B4) LS-C74900
Mouse Monoclonal [clone 16B4] (IgM,k)
Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-Phosphoserine Antibody LS-C210227
Rabbit Polyclonal
IHC, Western blot, Immunoprecipitation, ELISA
Unconjugated
Western blot analysis of the phosphorylated proteins with UV-treated cell lysates mouse spleen cell. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1 uM okadaic acid).

100% Guaranteed
Rabbit Polyclonal (IgG)
IHC, Western blot, Immunoprecipitation, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Phosphoserine
Rabbit
IgG Polyclonal
Unconjugated. Also available conjugated with HRP, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC (1:50)
  • Western blot
  • Immunoprecipitation
  • ELISA (1:250 - 1:1000)
  • (applications tested for the base form of this product only)

Specificity and Use

Phosphoserine antibody was raised against phosphoserine conjugated with KLH.
Recognizes phosphoserine, peptidyl-phosphoserine and serine-phosphorylated proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein. Species cross-reactivity: All species.
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and ELISA. Western Blot (tissue lysates): 50-100 ug/lane total protein, antibody dilution can be from 1:250-1:1000, overloading will show solid exposed lanes. Dilute the primary antibody until multiple phosphorylated bands will appear. If using purified proteins dilutions from 1:50-1:1000 will need to be tried. At 1:300 dilution the antibody normally detects 10 ng of protein with 2 hour incubations and enhanced HRP detection. Blocking of the blot should be with non-phosphoserine containing compounds such as 1-5% BSA in TBS that has been filtered through a 0.45um. Milk or casein is not recommended because of the presence of phospho-serine in these solutions. Buffers should be Tris based so that phosphate ion interference is minimized. Immunoprecipitation (tissue extracts): 10-20 ug/mg protein. Note that this will immunoprecipitate all phosphoserine proteins in the extracts. If using purified protein exacts containing only the protein of interest, antibody amounts should be decreased to 5 ug/500 ul of extracts. ELISA (kinase assay): 1:250-1:500. Immunohistochemistry: 1:50. Detects all phosphoserines, and any phosphorylated serine protein or peptide can be used to block antibody staining. Typically, a10M excess of peptide is used in Tris based buffers. Positive control: NIH 3T3 cells (+/- TPA), K562 cells and EGF-stimulated A431 cells.

Packaging

PBS, 50% glycerol.
For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.
For research use only.

About Phosphoserine


Requested From: 
Date Requested: 5/25/2018

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