LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-Phosphoserine Antibody LS-C74890

Ordering

Wt. Vol. Conc. Price
50 µg - 0.25 mg/ml $545
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Phosphoserine Antibodies

Anti-Phosphoserine Antibody LS-C74890
Rabbit Polyclonal (IgG)
IHC, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-Phosphoserine Antibody LS-C74891
Mouse Monoclonal (IgG1)
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG)
IHC, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Phosphoserine
Rabbit
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC (1:50)
  • Western blot
  • Immunoprecipitation
  • ELISA (1:250 - 1:1000)

Specificity and Use

Phosphoserine antibody was raised against phosphoserine conjugated with KLH.
Recognizes phosphoserine, peptidyl-phosphoserine and serine-phosphorylated proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein. Species cross-reactivity: All species.
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and ELISA. Western Blot (tissue lysates): 50-100 ug/lane total protein, antibody dilution can be from 1:250-1:1000, overloading will show solid exposed lanes. Dilute the primary antibody until multiple phosphorylated bands will appear. If using purified proteins dilutions from 1:50-1:1000 will need to be tried. At 1:300 dilution the antibody normally detects 10 ng of protein with 2 hour incubations and enhanced HRP detection. Blocking of the blot should be with non-phosphoserine containing compounds such as 1-5% BSA in TBS that has been filtered through a 0.45um. Milk or casein is not recommended because of the presence of phospho-serine in these solutions. Buffers should be Tris based so that phosphate ion interference is minimized. Immunoprecipitation (tissue extracts): 10-20 ug/mg protein. Note that this will immunoprecipitate all phosphoserine proteins in the extracts. If using purified protein exacts containing only the protein of interest, antibody amounts should be decreased to 5 ug/500 ul of extracts. ELISA (kinase assay): 1:250-1:500. Immunohistochemistry: 1:50. Detects all phosphoserines, and any phosphorylated serine protein or peptide can be used to block antibody staining. Typically, a10M excess of peptide is used in Tris based buffers. Positive control: NIH 3T3 cells (+/- TPA), K562 cells and EGF-stimulated A431 cells.

Packaging

PBS, 50% glycerol.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C.
For research use only.

About Phosphoserine


Requested From: United States
Date Requested: 12/6/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number