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Anti-PHGDH Antibody (clone 4A3-1D6) IHC-plus™ LS-B5457

Note: This antibody replaces LS-C133714

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PHGDH Antibodies

Anti-PHGDH Antibody (aa362-411) LS-C81937
Rabbit Polyclonal (IgG) to Human PHGDH
Human, Monkey, Mouse, Rat, Hamster
Western blot
Unconjugated
Western blot Image
Anti-PHGDH Antibody (clone 4A3-1D6) IHC-plus™ LS-B5457
Mouse Monoclonal [clone 4A3-1D6] (IgG1,k) to Human PHGDH
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 4A3-1D6] (IgG1,k) to Human PHGDH
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human PHGDH
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [4A3-1D6]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (1 - 10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA

Specificity and Use

PHGDH antibody was raised against pHGDH (AAH11262, 1 a.a. ~ 534 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human PHGDH
Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Tissue lysate, Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.4. Sourced in Ascites.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About PHGDH

O43175 NM_006623 NP_006614.2

PHGDH Antibody, 3-PGDH Antibody, 3PGDH Antibody, PGDH Antibody, Phosphoglycerate dehydrogenase Antibody, PDG Antibody, PGAD Antibody, PGDH3 Antibody

PHGDH encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

Immunohistochemistry

Immunohistochemistry
Anti-PHGDH antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5457 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to PHGDH on HeLa cell. [antibody concentration 1 ~10 ug/ml].

Western blot

Western blot
Western blot of PHGDH expression in human liver with PHGDH monoclonal antibody, clone 4A3-1D6.

Western blot

Western blot
Western blot of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody, clone 4A3-1D6.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation of PHGDH transfected lysate using anti-PHGDH monoclonal antibody and Protein A Magnetic Bead.
 

Requested From: United States
Date Requested: 12/10/2016

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