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Anti-PHAP1 / ANP32A Antibody (C-Terminus) IHC-plus™ LS-B3195

Note: This antibody replaces LS-C333

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PHAP1 / ANP32A Antibodies

Anti-PHAP1 / ANP32A Antibody (C-Terminus) LS-C431
Rabbit Polyclonal to Human PHAP1 / ANP32A
Human, Mouse
ICC, Western blot
Unconjugated
Immunocytochemistry Image
Anti-PHAP1 / ANP32A Antibody (C-Terminus) IHC-plus™ LS-B133
Rabbit Polyclonal to Human PHAP1 / ANP32A
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PHAP1 / ANP32A Antibody (aa121-170) IHC-plus™ LS-B10851
Rabbit Polyclonal (IgG) to Human PHAP1 / ANP32A
Human, Monkey, Bovine, Dog, Guinea pig, Horse, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human PHAP1 / ANP32A
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human PHAP1 / ANP32A
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • ELISA

Specificity and Use

PHAP1 / ANP32A antibody was raised against synthetic peptide from human ANP32A.
LS-E30024 - Liquid - 50 µg - $145.00
C-Terminus
PHAP antibody can be used for detection of PHAP I and PHAP III.
Immunohistochemistry: LS-B3195 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3195 was determined to be 5 ug/ml. Western Blot: Can be used for detection of PHAP I and PHAP III by Western blot at 0.5 to 2 ug/ml. At approximately 35 kD and 32 kD a band can be detected.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About PHAP1 / ANP32A

P39687 NM_006305 NP_006296.1

ANP32A Antibody, Hepatopoietin Cn Antibody, I1PP2A Antibody, LANP Antibody, HPPCn Antibody, PHAP1 Antibody, PHAPI Antibody, MAPM Antibody, PP32 Antibody, C15orf1 Antibody, Mapmodulin Antibody

Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis.

Immunohistochemistry

Immunohistochemistry
Anti-ANP32A antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3195 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-ANP32A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3195 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-ANP32A antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3195 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of PHAP in human small intestine tissue with PHAP antibody at 20 ug/ml.

Western blot

Western blot
Western blot of PHAP expression in human Raji cell lysate with PHAP antibody at 1 ug/ml. The two bands below PHAP I might be differently spliced isoforms of PHAP.
 

Requested From: United States
Date Requested: 12/8/2016

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