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Anti-PFKM / PFK-1 Antibody (C-Terminus) LS-C135936

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

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57 PFKM / PFK-1 Antibodies

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Anti-PFKM / PFK-1 Antibody (aa320-369) IHC-plus™ LS-B12566
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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human PFKM / PFK-1
Human, Mouse, Rat, Bat, Hamster, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human PFKM / PFK-1
Rabbit
Human, Mouse, Rat, Bat, Hamster, Zebrafish (tested or 100% immunogen sequence identity)
Monkey, Bovine, Dog, Guinea pig, Horse, Pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

PFKM / PFK-1 antibody was raised against synthetic peptide from C-Terminus of human PFKM (P08237, NP_000280). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Bat (100%); Monkey, Dog, Bovine, Horse, Pig, Guinea pig (92%); Elephant, Rabbit, Opossum, Chicken, Lizard, Xenopus, Salmon, Zebrafish (85%); Stickleback (84%).
LS-E22365 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135936. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
C-Terminus
Human PFKM / PFK-1
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About PFKM / PFK-1

P08237 NM_000289 NP_000280.1

PFKM Antibody, 6-phosphofructo-1-kinase Antibody, GSD7 Antibody, PFK1 Antibody, PFKA Antibody, PFKX Antibody, Phosphofructokinase-M Antibody, Phosphofructokinase 1 Antibody, PFK-1 Antibody, PFK-A Antibody, Phosphofructokinase, muscle Antibody, Phosphohexokinase Antibody

Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease.

Western blot

Western blot
PFKM / PFK-1 antibody LS-C135936 Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/23/2017

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