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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PFKFB2 Antibody (C‑Terminus) LS‑C135390
PFKFB2 antibody LS-C135390 is an unconjugated rabbit polyclonal antibody to PFKFB2 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C135390-50
50 µg
Unavailable

Popular PFKFB2 Products

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
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Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
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Species: Human, Mouse, Rat, Bovine
Applications: Western blot
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Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
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Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA

Product Description

PFKFB2 antibody LS-C135390 is an unconjugated rabbit polyclonal antibody to PFKFB2 from human, mouse, rat and other species. Validated for WB.
About PFKFB2
PFKFB2 is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. O60825 NM_006212 NP_006203.2

PFKFB2 Antibody, 6PF-2-K/Fru-2,6-P2ase 2 Antibody, PFK-2/FBPase-2 Antibody, PFK/FBPase 2 Antibody, PFKFB, cardiac Antibody


Specifications

Target
Mouse PFKFB2
Host
Rabbit
Reactivity
Mouse, Human, Monkey, Rat, Bat, Dog, Guinea pig, Horse, Pig, Rabbit, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Bovine (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
PFKFB2 antibody was raised against synthetic peptide from C-Terminus of mouse Pfkfb2 (Q6GTL7, NP_032851). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Xenopus (100%); Galago, Bovine (92%).
Blocking Peptide
LS-E20612 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135390. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Mouse PFKFB2
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

PFKFB2 antibody LS-C135390 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PFKFB2 antibody LS-C135390 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PFKFB2 antibody LS-C135390 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PFKFB2 antibody LS-C135390 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PFKFB2 antibody LS-C135390 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PFKFB2 antibody LS-C135390 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PFKFB2 antibody LS-C135390 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PFKFB2 antibody LS-C135390 Western blot of Mouse Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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