Human PECAM-1 / CD31

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Unconjugated

Tissue culture supernatant

Unmodified

Membrane preparation of a spleen from a patient with hairy cell leukemia. Cellular Localization: Cell membrane.

Recognizes human. endothelial cells in normal tissues and in benign and malignant proliferations 100kD (endothelial cells) and 130kD (platelets). In cryostat sections and blood smears the antibody also stains megakaryocytes, platelets and occasionally plasma cells. It reacts weakly with mantle zone B cells, peripheral T cells, and neutrophils. Antibody to CD31 is of value in the study of benign and malignant vascular tumors. Staining for CD31 has also been used to measure angiogenesis, which reportedly predicts tumor recurrence. Species cross-reactivity: Does not react with rat or porcine.

• IHC
• IHC - Paraffin (1:50)
• Immunofluorescence
• ELISA

Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry. Not suitable for Western Blot. Immunohistochemistry (Formalin/paraffin): 1:50 for 30min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0 for 10-20 min., followed by cooling at RT for 20 min. Positive control: Placenta.

PBS, 0.09% sodium azide, 40% glycerol.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16284 NCBI: NM_000442 NP_000433.4