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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PECAM‑1 / CD31 Antibody (clone 2C6) LS‑C173974
CD31 antibody LS-C173974 is an unconjugated mouse monoclonal antibody to human CD31 (PECAM-1). Validated for IHC and WB.
Catalog
Size
Price
LS-C173974-100
100 µl (1 mg/ml)
Unavailable

Popular PECAM-1 / CD31 Products

IHC of rat spleen cryosection with Mouse anti-Rat CD31 (LS-C43483).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Rat
Applications: IHC, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Anti-CD31 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2364 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-CD31 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4737 concentration 10 ug/ml.
Species: Mouse, Human
Applications: IHC, IHC - Paraffin
Anti-CD31 antibody IHC of human tonsil, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5577 dilution 1:100.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-PECAM-1 / CD31 antibody IHC staining of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10502 concentration 20 ug/ml.
Species: Human, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

CD31 antibody LS-C173974 is an unconjugated mouse monoclonal antibody to human CD31 (PECAM-1). Validated for IHC and WB.
About PECAM-1 / CD31
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. P16284 NM_000442 NP_000433.4

PECAM1 Antibody, CD31 Antibody, CD31 antigen Antibody, EndoCAM Antibody, PECA1 Antibody, PECAM-1 Antibody, GPIIA Antibody


Specifications

Target
Human PECAM-1 / CD31
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [2C6]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PECAM-1 / CD31 antibody was raised against full length human recombinant protein of human PECAM1(NP_000433) produced in HEK293T cell.
Specificity
Human CD31
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human Ovary tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Ovary tissue using anti-PECAM1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human colon tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Human colon tissue using anti-PECAM1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human Ovary tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Ovary tissue using anti-PECAM1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human colon tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Human colon tissue using anti-PECAM1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human Ovary tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Ovary tissue using anti-PECAM1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human colon tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Human colon tissue using anti-PECAM1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PECAM1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human Ovary tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Ovary tissue using anti-PECAM1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human colon tissue using anti-PECAM1 mouse monoclonal antibody.
IHC of paraffin-embedded Human colon tissue using anti-PECAM1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1.


Requested From: 
Date Requested: 6/22/2018

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