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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PDK1 Antibody (C‑Terminus, DY550) LS‑C141007
PDK1 antibody LS-C141007 is a DY550-conjugated rabbit polyclonal antibody to PDK1 from human, mouse and rat. Validated for IHC and WB.
Catalog
Size
Price
LS-C141007-100
100 µl
Unavailable

Popular PDK1 Products

Antibody
Species: Human, Monkey, Mouse, Rat, Dog, Pig
Applications: IHC, IHC - Paraffin, Western blot
Anti-PDK1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B689 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Anti-PDK1 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1733 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Anti-PDK1 antibody IHC of human skeletal muscle. human. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3690 concentration 10 ug/ml.
Species: Human, Monkey, Mouse, Rat, Dog, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA

Product Description

PDK1 antibody LS-C141007 is a DY550-conjugated rabbit polyclonal antibody to PDK1 from human, mouse and rat. Validated for IHC and WB.
About PDK1
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Q15118 NM_002610 NP_002601.1

PDK1 Antibody, PDHK Antibody, PDH kinase 1 Antibody, PDHK1 Antibody


Specifications

Target
Human PDK1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
DyLight 550. Also available Unconjugated or conjugated with Biotin, DY650, DY488.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (2 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PDK1 antibody was raised against a synthetic peptide made to a C-Terminal portion of the human PDK1 protein sequence (between residues 350-436). [Swiss-Prot Q15118] Percent identity by BLAST analysis: Mouse, Rat (100%).
Epitope
C-Terminus
Specificity
PDK1.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM sodium borate
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-PDK1 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1733 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PDK1 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1733 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of PDK1 in human heart lysate using PDK1 Antibody LS-B1733 at 2 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of PDK1 in human heart lysate using PDK1 Antibody LS-B1733 at 2 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of PDK1 in human heart lysate using antibody at 2 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of PDK1 in human heart lysate using antibody at 2 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-PDK1 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1733 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PDK1 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1733 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of PDK1 in human heart lysate using PDK1 Antibody LS-B1733 at 2 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of PDK1 in human heart lysate using PDK1 Antibody LS-B1733 at 2 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of PDK1 in human heart lysate using antibody at 2 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of PDK1 in human heart lysate using antibody at 2 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-PDK1 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1733 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PDK1 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1733 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of PDK1 in human heart lysate using PDK1 Antibody LS-B1733 at 2 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of PDK1 in human heart lysate using PDK1 Antibody LS-B1733 at 2 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of PDK1 in human heart lysate using antibody at 2 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of PDK1 in human heart lysate using antibody at 2 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-PDK1 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1733 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-PDK1 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1733 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of PDK1 in human heart lysate using PDK1 Antibody LS-B1733 at 2 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of PDK1 in human heart lysate using PDK1 Antibody LS-B1733 at 2 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of PDK1 in human heart lysate using antibody at 2 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of PDK1 in human heart lysate using antibody at 2 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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