Target
Human PDCD1 / CD279 / PD-1
Synonyms
PDCD1 | CD279 | HPD-1 | HPD-l | PD1 | Protein PD-1 | SLEB2 | PD-1 | CD279 antigen | Programmed cell death 1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Biotin.
Also available
Unconjugated.
Purification
Protein A purified
Immunogen
Recombinant human CD279.
Specificity
Anti-CD279 antibody clone ANC4H6 binds to CD279 present on PHA activated PBMC in FACS, and binds to recombinant CD279 in EIA. Pre incubation of recombinant CD279-coated plates with ANC4H6 blocks binding of biotinylated recombinant CD273 in EIA.
Applications
- Flow Cytometry
- Applications tested for the base form of this product only
Usage
Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated 5 days in culture with 5 ug/ml Phytohemagglutanin (PHA). Five x 105 cells per tube were pre incubated 10 minutes with 250 ug/ml human IgG (to block nonspecific binding) after which they were incubated 45 minutes on ice with 80 ul of product at a concentration of 10 ug/ml. Cells were then washed twice and incubated 45 minutes with 20 ul of 2o reagent Streptavidin/R-PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.04 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when reagent was pre incubated 10 minutes with a ten-fold excess of recombinant CD279-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
Storage
Store at 4°C, avoid freezing and store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDCD1 / CD279 / PD-1