Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 4F12] (IgG2b,k) to Human PDCD1 / CD279 / PD-1
Western blot, ELISA
Human PDCD1 / CD279 / PD-1
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [4F12]
Protein G purified
Western blot (1:500 - 1:1000)
IHC - Paraffin
Specificity and Use
PDCD1 / CD279 / PD-1 antibody was raised against recombinant human PDCD1 (21-167aa) purified from E. coli.
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since applications vary, each investigator should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500-1:1000.
PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
PDCD1 / CD279 / PD-1 is a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure.
Cell lysates of 293T and U937 (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Requested From: United States
Date Requested: 10/22/2016