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Anti-PCNA Antibody LS-C9526

Ordering

Wt. Vol. Conc. Price
100 µg - - $475
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Mouse Monoclonal (IgG2a) to Human PCNA
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human PCNA
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal
Protein A purified

Applications

IHC - Paraffin, Immunofluorescence, Western blot (1:250 - 1:1000), Immunoprecipitation, Flow Cytometry

Specificity and Use

PCNA antibody was raised against rat PCNA made in the protein A vector of pR1T2T.
Recognizes PCNA from all vertebrate and insect species tested and has been used to identify transformed cells, proliferating cells in solid tumors, and blast cells in leukemia patients. Species cross-reactivity: Invertebrates and vertebrates.
Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, and Indirect Flow Cytometry. Western Blot: 1:250-1:1000, Nuclear extracts are preferred over whole cell protein extracts. Detects a polypeptide at 36kD with an isoelectric point of 4.8. Immunohistochemistry (paraffin embedded tissue sections only): 1:20-1:200, may be used on formalin (buffered or unbuffered), methacarn and Bouin's reagent. The time of fixation can markedly affect the intensity of PCNA immunoreactivity. Staining is reduced (and may be abolished) if sections are baked onto glass slides. Air drying overnight on poly-L-lysine coated slides is recommended. Incubate for 1 hour at 25?C using standard ABC. Positive control: Tonsil or reactive lymph node.

Packaging

PBS, pH 7.2, 1 mg/ml BSA, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About PCNA

P12004 NM_002592 NP_002583.1

PCNA Antibody, Cyclin Antibody

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

Requested From: United States
Date Requested: 9/26/2016

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