LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
PCNA Antibody (clone 3A3) LS‑C175498
PCNA antibody LS-C175498 is an unconjugated mouse monoclonal antibody to human PCNA. Validated for IHC and WB.
Catalog
Size
Price
LS-C175498-100
100 µl (1 mg/ml)
Unavailable

Popular PCNA Products

Anti-PCNA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B402 concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Chicken, Xenopus, Fish
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-PCNA antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1887 concentration 10 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Anti-PCNA antibody IHC of human colon, MALT. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2826 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Anti-PCNA antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3434 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat, Human, Mouse, Chicken, Zebrafish, Drosophila, Yeast, Primate
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Anti-PCNA antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6747 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

PCNA antibody LS-C175498 is an unconjugated mouse monoclonal antibody to human PCNA. Validated for IHC and WB.
About PCNA
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. P12004 NM_002592 NP_002583.1

PCNA Antibody, Cyclin Antibody


Specifications

Target
Human PCNA
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [3A3]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PCNA antibody was raised against full length human recombinant protein of human PCNA (NP_002583) produced in E. coli.
Specificity
Human PCNA / Cyclin
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PCNA mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PCNA mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCNA.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCNA.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PCNA mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PCNA mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCNA.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCNA.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PCNA mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PCNA mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCNA.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCNA.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PCNA mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PCNA mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCNA.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCNA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCNA.


Requested From: 
Date Requested: 6/21/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number